Chemical fact sheet: Quercetin 3-O-galactoside

STD

True

TLC

False

UV/Vis detector description

HPLC-DAD

Mass spectrometer description

UHPLC-QTOF-MS

Organism

Salix pyrolifolia

dried buds, fresh, dried

Sample note

The researchers collected S. pyrolifolia samples from different -aged trees of the same clone grown in Kaavi, Finland.

Extraction solvents

cold methanol

Extraction repeats

5

Extract drying method

evaporation in vacuo

Analysis solvents

water:MeOH, 1:1

Detection note

Na+ -adduct = 23.98977; 487.0852-23.98977 = 463.09543; the compound was detected in the barks of all studied ages (1, 3 and 20 years)

References

A. Lavola, M. Maukonen, and R. Julkunen-Tiitto, "Variability in the composition of phenolic compounds in winter-dormant Salix pyrolifolia in relation to plant part and age," Phytochemistry , vol. 153 , pp. 102–110 , DOI: 10.1016/j.phytochem.2018.05.021 .

STD

True

TLC

False

UV/Vis detector description

UV/Vis

Mass spectrometer description

UPLC-ESI-Q-TOF-MS/MS

Organism

Rheum officinale  Baill.

cultivated

fresh, powdered

Sample note

The identification of Rheum officinale from the collected rhizome specimens were conducted by Prof. Li Xiang at the Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, China.

Dried material storage temperature

-80 °C

Extraction solvents

methanol

Extraction repeats

1

Extraction temperature

4 °C

Analysis solvents

methanol

References

J. Liu, L. Leng, Y. Liu, H. Gao, W. Yang, S. Chen, and A. Liu, "Identification and quantification of target metabolites combined with transcriptome of two rheum species focused on anthraquinone and flavonoids biosynthesis.," Scientific Reports , vol. 10 , no. 1 , pp. 20241 , DOI: 10.1038/s41598-020-77356-9 .

STD

True

TLC

False

UV/Vis detector description

UV/Vis

Mass spectrometer description

UPLC-ESI-Q-TOF-MS/MS

Organism

Rheum palmatum  L.

cultivated

fresh, powdered

Sample note

The identification of Rheum palmatum from the collected rhizome specimens were conducted by Prof. Li Xiang at the Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, China.

Dried material storage temperature

-80 °C

Extraction solvents

methanol

Extraction repeats

1

Extraction temperature

4 °C

Analysis solvents

methanol

References

J. Liu, L. Leng, Y. Liu, H. Gao, W. Yang, S. Chen, and A. Liu, "Identification and quantification of target metabolites combined with transcriptome of two rheum species focused on anthraquinone and flavonoids biosynthesis.," Scientific Reports , vol. 10 , no. 1 , pp. 20241 , DOI: 10.1038/s41598-020-77356-9 .

STD

True

TLC

False

UV/Vis detector description

UPLC-PDA

Mass spectrometer description

UPLC-PDA-ESI-MS/MS, UPLC-QTOF-MS

Organism

Aronia melanocarpa 'Galicjanka'

cultivated

ground, dried, passed through a strainer (1mm)

Sample note

The fruit samples (about 15kg) were obtained from a horticultural farm in Trzebnica, near Wroclaw, Poland. The raw material was collected at the optimum ripening stage recommended for consumption. The whole fruits were freeze-dried, so that the pressure was reduced to 0.0960 kPa. The temperature in the drying chamber was -60 C, and in the shelves 26C. The dried material was ground with laboratory mill (IKA A.11, Christ) and then passed through a strainer (1mm). The powder (code PDF) was ready for the analyses.

Drying methods

freeze-dried

Drying temperature

26 °C

Extraction solvents

methanol acidified with 2 % formic acid

Extraction mass/volume-ratio

40 mg/mL

Extraction repeats

2

Extraction time

30 min

Analysis solvents

methanol acidified with 2 % formic acid

References

J. Oszmiański, and S. Lachowicz, "Effect of the production of dried fruits and juice from chokeberry (Aronia melanocarpa L.) on the content and antioxidative activity of bioactive compounds.," Molecules , vol. 21 , no. 8 , pp. 1098 , DOI: 10.3390/molecules21081098 .

STD

True

TLC

False

UV/Vis detector description

UPLC-PDA

Mass spectrometer description

UPLC-PDA-ESI-MS/MS, UPLC-QTOF-MS

Organism

Aronia melanocarpa 'Galicjanka'

cultivated

pressed, dried, passed through a strainer (1mm), ground

Sample note

The fruit samples (about 15kg) were obtained from a horticultural farm in Trzebnica, near Wroclaw, Poland. The raw material was collected at the optimum ripening stage recommended for consumption. The whole, uncrushed fruits were pressed on a hydraulic press (SSRE, Waesaw, Poland). The obtained pomace was freeze-dried using an Alpha 1-4 LSC freeze dryer. The pressure was reduced to 0.960kPa. The temperature in the drying chamber was -60 C, and in the shelves 26C.Then, the material was ground, then passed through a strainer (1mm). After that the powder (code PPUF) was ready for the analyses.

Drying methods

freeze-dried

Drying temperature

26 °C

Extraction solvents

methanol acidified with 2 % formic acid

Extraction mass/volume-ratio

40 mg/mL

Extraction repeats

2

Extraction time

30 min

Analysis solvents

methanol acidified with 2 % formic acid

References

J. Oszmiański, and S. Lachowicz, "Effect of the production of dried fruits and juice from chokeberry (Aronia melanocarpa L.) on the content and antioxidative activity of bioactive compounds.," Molecules , vol. 21 , no. 8 , pp. 1098 , DOI: 10.3390/molecules21081098 .

STD

True

TLC

False

UV/Vis detector description

HPLC-PDA

Mass spectrometer description

ESI-MS

Organism

Calluna vulgaris  (L.) Hull

wild

ground, dried

Collection dates

2004, 2005

Sample note

The samples were collected from the Naturpark Sölktäler.

Drying methods

air-dried

Dried material storage temperature

15 °C

Dried material storage notes

dark; in the brown glass bottles

Extraction solvents

80 % methanol

Extraction mass/volume-ratio

91 mg/mL

Extraction repeats

1

Extraction temperature

60 °C

Analysis solvents

MeOH:water, 8:2

References

G. Rieger, M. Müller, H. Guttenberger, and F. Bucar, "Influence of altitudinal variation on the content of phenolic compounds in wild populations of Calluna vulgaris, Sambucus nigra, and Vaccinium myrtillus.," Journal of Agricultural and Food Chemistry , vol. 56 , no. 19 , pp. 9080–9086 , DOI: 10.1021/jf801104e .

STD

True

TLC

False

UV/Vis detector description

HPLC-DAD, HPLC-PDA

Mass spectrometer description

LC-ESI-MS

Organism

Filipendula ulmaria  (L.) Maxim.

wild

ground, dried

Collection dates

2016-7

Sample note

Plant material was identified by Professor Branislava Lakušik (Department of Botany, University of Belgrade - Faculty of Pharmacy) and voucher specimen; number 3872HFF was deposited in the Herbarium of the Department of Botany, University of Belgrade - Faculty of Pharmacy.

Extraction solvents

boiling water

Extraction repeats

1

Extraction time

30 min

Extract drying method

freeze-drying

Analysis solvents

aqueous infusion

Detection note

The compound was not detected; the UV spectral data was not shown.

References

S. Samardžić, J. Arsenijević, D. Božić, M. Milenković, V. Tešević, and Z. Maksimović, "Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.," Journal of Ethnopharmacology , vol. 213 , pp. 132–137 , DOI: 10.1016/j.jep.2017.11.013 .

STD

True

TLC

False

UV/Vis detector description

HPLC-DAD, HPLC-PDA

Mass spectrometer description

LC-ESI-MS

Organism

Filipendula vulgaris  Moench

wild

ground, dried

Collection dates

2013-5, 2014-5

Sample note

Plant material was collected in 2013 and 2014 (analysed together) near Loćika and identified by Professor Branislava Lakušik (Department of Botany, University of Belgrade - Faculty of Pharmacy) and voucher specimen; number 3713HFF was deposited in the Herbarium of the Department of Botany, University of Belgrade - Faculty of Pharmacy.

Extraction solvents

boiling water

Extraction repeats

1

Extraction time

30 min

Extract drying method

freeze-drying

Analysis solvents

aqueous infusion

Detection note

The compound was quantified; the UV spectral data was not shown.

References

S. Samardžić, J. Arsenijević, D. Božić, M. Milenković, V. Tešević, and Z. Maksimović, "Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.," Journal of Ethnopharmacology , vol. 213 , pp. 132–137 , DOI: 10.1016/j.jep.2017.11.013 .

STD

True

TLC

False

UV/Vis detector description

UHPLC

Mass spectrometer description

UHPLC-MS, HRMS, LTQ OrbiTrap, UHPLC–LTQ OrbiTrap MS/MS, HESI, heated ESI

Organism

Tanacetum parthenium  (L.) Sch. Bip.

wild

ground, dried

Sample note

The samples were collected in Turkey (Taskopru, Karacaoglu village). Taxonomic spotting was performed at Marmara University, Istanbul, Turkey, voucher number: MARE-19056./ Microwave-assisted extraction (MAE) was performed at 600W microwave power.

Drying methods

air-dried

Extraction solvents

ethanol

Extraction mass/volume-ratio

50 mg/mL

Extraction repeats

1

Extraction time

30 min

Extract drying method

concentration under vacuum

Extract drying temperature

40 °C

Dried extract storage temperature

4 °C

Detection note

MS2 fragments (% base peak): 301 (100), 300 (30); MS3: 273 (25), 257 (20), 179 (100), 151 (75); MS4: 151 (100)

References

G. Zengin, A. Cvetanonović, U. Gašić, A. Stupar, G. Bulut, I. Şenkardes, A. Dogan, K. Sinan, Z. Aumeeruddy-Elalfi, A. Aktumsek, and M. Mahomoodally, "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch. Bip.," Industrial Crops and Products , vol. 146 , pp. 112202 , DOI: 10.1016/j.indcrop.2020.112202 .

STD

True

TLC

False

UV/Vis detector description

UHPLC

Mass spectrometer description

UHPLC-MS, HRMS, LTQ OrbiTrap, UHPLC–LTQ OrbiTrap MS/MS, HESI, heated ESI

Organism

Tanacetum parthenium  (L.) Sch. Bip.

wild

ground, dried

Sample note

The samples were collected in Turkey (Taskopru, Karacaoglu village). Taxonomic spotting was performed at Marmara University, Istanbul, Turkey, voucher number: MARE-19056./Sonication of plant-ethanol mixture was done in ultrasonic bath for an hour at 30 °C.

Drying methods

air-dried

Extraction solvents

ethanol

Extraction mass/volume-ratio

40 mg/mL

Extraction repeats

1

Extraction time

1 h

Extraction temperature

30 °C

Extract drying method

concentration under vacuum

Extract drying temperature

40 °C

Dried extract storage temperature

4 °C

Detection note

MS2 fragments (% base peak): 301 (100), 300 (30); MS3: 273 (25), 257 (20), 179 (100), 151 (75); MS4: 151 (100)

References

G. Zengin, A. Cvetanonović, U. Gašić, A. Stupar, G. Bulut, I. Şenkardes, A. Dogan, K. Sinan, Z. Aumeeruddy-Elalfi, A. Aktumsek, and M. Mahomoodally, "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch. Bip.," Industrial Crops and Products , vol. 146 , pp. 112202 , DOI: 10.1016/j.indcrop.2020.112202 .

STD

True

TLC

False

UV/Vis detector description

UHPLC

Mass spectrometer description

UHPLC-MS, HRMS, LTQ OrbiTrap, UHPLC–LTQ OrbiTrap MS/MS, HESI, heated ESI

Organism

Tanacetum parthenium  (L.) Sch. Bip.

wild

ground, dried

Sample note

The samples were collected in Turkey (Taskopru, Karacaoglu village). Taxonomic spotting was performed at Marmara University, Istanbul, Turkey, voucher number: MARE-19056./The plant samples were macerated at room temperature at dark for 24 h.

Drying methods

air-dried

Extraction solvents

ethanol

Extraction mass/volume-ratio

50 mg/mL

Extraction repeats

1

Extraction time

1 d

Extraction temperature

20±5 °C

Extract drying method

concentration under vacuum

Extract drying temperature

40 °C

Dried extract storage temperature

4 °C

Detection note

MS2 fragments (% base peak): 301 (100), 300 (30); MS3: 273 (25), 257 (20), 179 (100), 151 (75); MS4: 151 (100)

References

G. Zengin, A. Cvetanonović, U. Gašić, A. Stupar, G. Bulut, I. Şenkardes, A. Dogan, K. Sinan, Z. Aumeeruddy-Elalfi, A. Aktumsek, and M. Mahomoodally, "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch. Bip.," Industrial Crops and Products , vol. 146 , pp. 112202 , DOI: 10.1016/j.indcrop.2020.112202 .