Analysis result 1
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
463
|
m/z |
| MS²⁻ |
300
301
463
|
m/z |
UV/Vis detector description
Mass spectrometer description
ESI-MS/MS
Organism
Rhamnus davurica
Pall.
Sample note
The authentication and identification of the barks of Rhamnus davurica was performed with the researchers of this study which were assisted by the taxonomist Quangwan Hu from Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture (Wuhan Botanicl Garden), Chinese Academy of Sciences. A voucher specimen (No. 0031) was deposited in the herbarium of the Key Laboratory.
Dried material storage temperature
4 °C
Dried material storage notes
the samples were packed in sealed polyethylene bags; stored in a refrigerator until use; dark
Extraction solvents
60 % ethanol
Extraction time
1 h 30 min
Extraction temperature
20±5 °C
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
References
G.
Chen,
X.
Li,
F.
Saleri,
and
M.
Guo,
"Analysis of flavonoids in Rhamnus davurica and its antiproliferative effects.,"
Molecules
,
vol. 21
,
no. 10
,
pp. 1275
,
DOI: 10.3390/molecules21101275
.
Analysis result 2
| Detection technique |
Values |
Units |
| UV/Vis |
250
344
|
nm |
| [M⁻ H]⁻ |
463.08700
|
m/z |
UV/Vis detector description
LC-diode array (DAD)
Mass spectrometer description
ESI-TOF-MS
Organism
Tanacetum vulgare
L.
Sample note
The plant was authenticated by the authors; rev.: Dr. Goran Anackov and prepared as herbarium specimens and were deposited at the Herbarium of the Department of Biology and Ecology-BUNS Herbariumm University of Novi Sad, voucher No. 2-2069.
Dried material storage temperature
15 °C
Dried material storage notes
dark; as whole
Extraction solvents
methanol
Extraction mass/volume-ratio
100 mg/mL
Extraction time
2 d 20 min
Extraction temperature
20±5 °C
Extract drying method
evaporation under vacuum
Extract drying temperature
40 °C
Dried extract storage temperature
20±5 °C
Detection note
Other, less abundant m/z values: 464.0907, 499.0642, 500.0684, 509.0931, 510.0968
References
N.
Devrnja,
B.
Anđelković,
S.
Aranđelović,
S.
Radulović,
M.
Soković,
D.
Krstić-Milošević,
M.
Ristić,
and
D.
Ćalić,
"Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essentiall oils and methanol extracts.,"
South African Journal of Botany
,
vol. 111
,
pp. 212–221
,
DOI: 10.1016/j.sajb.2017.03.028
.
Analysis result 3
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 4
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 5
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 6
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 7
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 8
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 9
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 10
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were yellow and the pistils became orange as the development proceeded, and the colour started to change gradually yellos/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 11
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791)) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to yellow&/red. The pistils became orange as the development proceeded.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 12
| Detection technique |
Values |
Units |
| UV/Vis |
256
354
|
nm |
| [M⁻ H]⁻ |
463.09633
487.08610
|
m/z |
UV/Vis detector description
HPLC-DAD
Mass spectrometer description
UHPLC-QTOF-MS
Organism
Salix pyrolifolia
Sample note
The researchers collected S. pyrolifolia samples from different -aged trees of the same clone grown in Kaavi, Finland.
Extraction solvents
cold methanol
Extract drying method
evaporation in vacuo
Analysis solvents
water:MeOH, 1:1
Detection note
Na+ -adduct = 23.98977; 487.0861-23.98977 = 463.09633; the compound was detected in the barks of all studied ages (1, 3 and 20 years)
References
A.
Lavola,
M.
Maukonen,
and
R.
Julkunen-Tiitto,
"Variability in the composition of phenolic compounds in winter-dormant Salix pyrolifolia in relation to plant part and age,"
Phytochemistry
,
vol. 153
,
pp. 102–110
,
DOI: 10.1016/j.phytochem.2018.05.021
.
Analysis result 13
| Detection technique |
Values |
Units |
| UV/Vis |
254
369
|
nm |
| [M⁻ H]⁻ |
463.09790
|
m/z |
| MS²⁻ |
355.11200
|
m/z |
UV/Vis detector description
HPLC-DAD
Mass spectrometer description
ESI-TOF-MS
Organism
Helianthus annuus
L.
Sample note
The researchers collected ray florets from the sunflower capitulum.
Drying methods
dried in the dark, air-dried
Dried material storage temperature
15 °C
Dried material storage notes
in a brown desiccator with oxygen scavenger
Extraction solvents
80 % acetone (free phenolic compounds)
Extraction mass/volume-ratio
10 mg/mL
Extract liquid storage temperature
-80 °C
Extract drying method
evaporation
Extract drying temperature
45 °C
References
Q.
Liang,
J.
Cui,
H.
Li,
J.
Liu,
and
G.
Zhao,
"Florets of sunflower (Helianthus annuus L.): potential new sources of dietary fiber and phenolic acids.,"
Journal of Agricultural and Food Chemistry
,
vol. 61
,
pp. 3435–3443
.
Analysis result 14
| Detection technique |
Values |
Units |
| UV/Vis |
254
369
|
nm |
| [M⁻ H]⁻ |
463.09790
|
m/z |
| MS²⁻ |
355.11200
|
m/z |
UV/Vis detector description
HPLC-DAD
Mass spectrometer description
ESI-TOF-MS
Organism
Helianthus annuus
L.
Sample note
The researchers collected disc florets from the sunflower capitulum.
Drying methods
dried in the dark, air-dried
Dried material storage temperature
15 °C
Dried material storage notes
in a brown desiccator with oxygen scavenger
Extraction solvents
80 % acetone (free phenolic compounds)
Extraction mass/volume-ratio
10 mg/mL
Extract liquid storage temperature
-80 °C
Extract drying method
evaporation
Extract drying temperature
45 °C
References
Q.
Liang,
J.
Cui,
H.
Li,
J.
Liu,
and
G.
Zhao,
"Florets of sunflower (Helianthus annuus L.): potential new sources of dietary fiber and phenolic acids.,"
Journal of Agricultural and Food Chemistry
,
vol. 61
,
pp. 3435–3443
.
Analysis result 15
| Detection technique |
Values |
Units |
| UV/Vis |
352
|
nm |
| [M⁻ H]⁻ |
463
|
m/z |
| MS²⁻ |
301
|
m/z |
UV/Vis detector description
UPLC-PDA
Mass spectrometer description
UPLC-PDA-ESI-MS/MS, UPLC-QTOF-MS
Organism
Aronia melanocarpa
'Galicjanka'
ground, dried, passed through a strainer (1mm)
Sample note
The fruit samples (about 15kg) were obtained from a horticultural farm in Trzebnica, near Wroclaw, Poland. The raw material was collected at the optimum ripening stage recommended for consumption. The whole fruits were freeze-dried, so that the pressure was reduced to 0.0960 kPa. The temperature in the drying chamber was -60 C, and in the shelves 26C. The dried material was ground with laboratory mill (IKA A.11, Christ) and then passed through a strainer (1mm). The powder (code PDF) was ready for the analyses.
Drying methods
freeze-dried
Extraction solvents
methanol acidified with 2 % formic acid
Extraction mass/volume-ratio
40 mg/mL
Analysis solvents
methanol acidified with 2 % formic acid
References
J.
Oszmiański,
and
S.
Lachowicz,
"Effect of the production of dried fruits and juice from chokeberry (Aronia melanocarpa L.) on the content and antioxidative activity of bioactive compounds.,"
Molecules
,
vol. 21
,
no. 8
,
pp. 1098
,
DOI: 10.3390/molecules21081098
.
Analysis result 16
| Detection technique |
Values |
Units |
| UV/Vis |
352
|
nm |
| [M⁻ H]⁻ |
463
|
m/z |
| MS²⁻ |
301
|
m/z |
UV/Vis detector description
UPLC-PDA
Mass spectrometer description
UPLC-PDA-ESI-MS/MS, UPLC-QTOF-MS
Organism
Aronia melanocarpa
'Galicjanka'
pressed, dried, passed through a strainer (1mm), ground
Sample note
The fruit samples (about 15kg) were obtained from a horticultural farm in Trzebnica, near Wroclaw, Poland. The raw material was collected at the optimum ripening stage recommended for consumption. The whole, uncrushed fruits were pressed on a hydraulic press (SSRE, Waesaw, Poland). The obtained pomace was freeze-dried using an Alpha 1-4 LSC freeze dryer. The pressure was reduced to 0.960kPa. The temperature in the drying chamber was -60 C, and in the shelves 26C.Then, the material was ground, then passed through a strainer (1mm). After that the powder (code PPUF) was ready for the analyses.
Drying methods
freeze-dried
Extraction solvents
methanol acidified with 2 % formic acid
Extraction mass/volume-ratio
40 mg/mL
Analysis solvents
methanol acidified with 2 % formic acid
References
J.
Oszmiański,
and
S.
Lachowicz,
"Effect of the production of dried fruits and juice from chokeberry (Aronia melanocarpa L.) on the content and antioxidative activity of bioactive compounds.,"
Molecules
,
vol. 21
,
no. 8
,
pp. 1098
,
DOI: 10.3390/molecules21081098
.
Analysis result 17
UV/Vis detector description
HPLC-PDA
Mass spectrometer description
ESI-MS
Organism
Calluna vulgaris
(L.) Hull
Collection dates
2004, 2005
Sample note
The samples were collected from the Naturpark Sölktäler.
Dried material storage temperature
15 °C
Dried material storage notes
dark; in the brown glass bottles
Extraction solvents
80 % methanol
Extraction mass/volume-ratio
91 mg/mL
Extraction temperature
60 °C
Analysis solvents
MeOH:water, 8:2
References
G.
Rieger,
M.
Müller,
H.
Guttenberger,
and
F.
Bucar,
"Influence of altitudinal variation on the content of phenolic compounds in wild populations of Calluna vulgaris, Sambucus nigra, and Vaccinium myrtillus.,"
Journal of Agricultural and Food Chemistry
,
vol. 56
,
no. 19
,
pp. 9080–9086
,
DOI: 10.1021/jf801104e
.
Analysis result 18
| Detection technique |
Values |
Units |
| UV/Vis |
ND
|
nm |
UV/Vis detector description
HPLC-DAD, HPLC-PDA
Mass spectrometer description
LC-ESI-MS
Organism
Filipendula ulmaria
(L.) Maxim.
Sample note
Plant material was identified by Professor Branislava Lakušik (Department of Botany, University of Belgrade - Faculty of Pharmacy) and voucher specimen; number 3872HFF was deposited in the Herbarium of the Department of Botany, University of Belgrade - Faculty of Pharmacy.
Extraction solvents
boiling water
Extract drying method
freeze-drying
Analysis solvents
aqueous infusion
Detection note
The compound was not detected; the UV spectral data was not shown.
References
S.
Samardžić,
J.
Arsenijević,
D.
Božić,
M.
Milenković,
V.
Tešević,
and
Z.
Maksimović,
"Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.,"
Journal of Ethnopharmacology
,
vol. 213
,
pp. 132–137
,
DOI: 10.1016/j.jep.2017.11.013
.
Analysis result 19
UV/Vis detector description
HPLC-DAD, HPLC-PDA
Mass spectrometer description
LC-ESI-MS
Organism
Filipendula vulgaris
Moench
Collection dates
2013-5, 2014-5
Sample note
Plant material was collected in 2013 and 2014 (analysed together) near Loćika and identified by Professor Branislava Lakušik (Department of Botany, University of Belgrade - Faculty of Pharmacy) and voucher specimen; number 3713HFF was deposited in the Herbarium of the Department of Botany, University of Belgrade - Faculty of Pharmacy.
Extraction solvents
boiling water
Extract drying method
freeze-drying
Analysis solvents
aqueous infusion
Detection note
The compound was quantified; the UV spectral data was not shown.
References
S.
Samardžić,
J.
Arsenijević,
D.
Božić,
M.
Milenković,
V.
Tešević,
and
Z.
Maksimović,
"Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.,"
Journal of Ethnopharmacology
,
vol. 213
,
pp. 132–137
,
DOI: 10.1016/j.jep.2017.11.013
.
Analysis result 20
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
463
|
m/z |
| MS²⁻ |
300
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Dimitrograd was no. 2-1765. The aerial parts were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z), respectively, are presented from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 21
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
463
: ND
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Dimitrograd was no. 2-1765. The bulbs were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
Quercetin 3-O-glucoside was not detected in the bulbs, contrary to the aerial parts of this plant taxa.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 22
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
463
|
m/z |
| MS²⁻ |
300
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Babusnica was no. 2-1767. The aerial parts were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z), respectively, are presented from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 23
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
463
|
m/z |
| MS²⁻ |
300
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Babusnica was no. 2-1767. The bulbs were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z), respectively, are presented from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 24
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
463
|
m/z |
| MS²⁻ |
300
|
m/z |
UV/Vis detector description
HPLC-UV/Vis
Mass spectrometer description
HPLC/ESI-ITMSn, electrospray ionization multistage ion trap mass spectrometry
Organism
Allium cepa
var. Ramata di Montoro
L.
Sample note
The variety is cultivated in a niche geographical area in southern Italy. The cultivated bulbs were provide by local committee promoters of Cipolla Ramata di Montoro located in Montoro (Campania, Italy).
Extraction solvents
80 % methanol
Extraction mass/volume-ratio
500 mg/mL
Extraction temperature
20±5 °C
Extract drying method
rotary evaporation
Dried extract storage temperature
-20 °C
Analysis solvents
1 % formic acid
References
I.
Tedesco,
V.
Carbone,
C.
Spagnuolo,
P.
Minasi,
and
G.
Russo,
"Identification and quantification of flavonoids from two southern Italian cultivars of Allium cepa L., Tropea (red onion) and Montoro (copper onion), and their capacity to protect human erythrocytes from oxidative stress.,"
Journal of Agricultural and Food Chemistry
,
vol. 63
,
pp. 5229–5238
,
DOI: 10.1021/acs.jafc.5b01206
.
Analysis result 25
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
463
|
m/z |
| MS²⁻ |
300
|
m/z |
UV/Vis detector description
HPLC-UV/Vis
Mass spectrometer description
HPLC/ESI-ITMSn, electrospray ionization multistage ion trap mass spectrometry
Organism
Allium cepa
var. Tropea
L.
Sample note
Tropea variety is a well known red onion in southern Italy. The researchers collected onion bulbs in Tropea, Calabria, Italy.
Extraction solvents
80 % methanol
Extraction mass/volume-ratio
500 mg/mL
Extraction temperature
20±5 °C
Extract drying method
rotary evaporation
Dried extract storage temperature
-20 °C
Analysis solvents
1 % formic acid
References
I.
Tedesco,
V.
Carbone,
C.
Spagnuolo,
P.
Minasi,
and
G.
Russo,
"Identification and quantification of flavonoids from two southern Italian cultivars of Allium cepa L., Tropea (red onion) and Montoro (copper onion), and their capacity to protect human erythrocytes from oxidative stress.,"
Journal of Agricultural and Food Chemistry
,
vol. 63
,
pp. 5229–5238
,
DOI: 10.1021/acs.jafc.5b01206
.
Analysis result 26
UV/Vis detector description
HPLC-DAD
Mass spectrometer description
ESI-TOF-MS
Organism
Helianthus annuus
L.
60-mesh screen, dried, pulverized
Sample note
The capitula of flowers (Helianthus annuus L.) were collected by the researchers. The capitula are agricultural byproducts of sunflower seeds.
Drying methods
air-dried, dried in the shade
Dried material storage temperature
15 °C
Dried material storage notes
in a brown desiccator with oxygen scavenger
Extraction solvents
50 % methanol, 90 % methanol, 50 % ethanol, 90 % ethanol
Extraction time
1 h 20 min
Extraction temperature
40 °C
Extract drying method
evaporation in vacuo
Extract drying temperature
45 °C
References
F.
Ye,
Q.
Liang,
H.
Li,
and
G.
Zhao,
"Solvent effects on phenolic content, composition, and antioxidant activity of etracts from florets of sunflower (Helianthus annuus L.),"
Industrial Crops and Products
,
vol. 76
,
pp. 574–581
,
DOI: 10.1016/j.indcrop.2015.07.063
.
