Analysis result 1
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 2
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 3
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
