Chemical fact sheet: Columbamine

Columbamine

Basics

Category
Protoberberine & other isoquinoline alkaloid derivatives
IUPAC-name
3,9,10-Trimethoxy-5,6-dihydroisoquinolino[2,1-b]isoquinolin-7-ium-2-ol
Formula
No formula stored
Exact mass
338.13870 g/mol
Molecular weight
No weights stored
Structure
Chemical structure of columbamine
Figure 1.1: Chemical structure of columbamine

Sources

In summary, the chemical columbamine has been analyzed from following sources:

Berberis petiolaris  Wall. ex G. Don
1st

Note that an analysis result in the database may indicate either presence or lack thereof of a chemical in an analyzed sample.

References

  1. A. Singh, V. Bajpai, M. Srivastava, K. Arya, and B. Kumar, "Rapid profiling and structural characterization of bioactive compounds and their distribution in different parts of Berberis petiolaris Wall. ex G. Don applying hyphenated mass spectrometric techniques," Rapid Communications in Mass Spectrometry , vol. 28 , no. 19 , pp. 2089-2100 , DOI: 10.1002/rcm.7001 .

Analysis results

Analysis result 1

Detection technique Values Units
M⁺ 338.13870 m/z
MS²⁺ 265.07390
279.08900
280.09850
294.11130
306.07620
308.09080
322.10730
327.11270 		m/z
STD
False
TLC
False
UV/Vis detector description
HPLC-UV
Mass spectrometer description
QTOF-ESI-MS/MS, QTRAP hybrid triple quadrupole-linear ion trap mass spectrometer
Organism
Berberis petiolaris  Wall. ex G. Don
wild
ground, dried
Sample note
The plant material was collected from Pandukholi Forest, Almora, Uttarakhand (India), and identified according to the Flora of District Garhwal, North-West Himalaya, the Forest Flora of Kumaon. A voucher specimen of B. petiolaris, KRA 24410, was deposited in the departmental Herbarium of CDRI. Each part of the plant (fruit, leaf, root and stem) was washed thoroughly under tap water and dried at room temperature.
Drying methods
air-dried
Drying temperature
20±5 °C
Extraction solvents
ethanol
Extraction mass/volume-ratio
40 mg/mL
Extraction repeats
5
Extraction time
2 h 30 min
Extraction temperature
30 °C
Extract liquid storage temperature
20±5 °C
Extract drying method
vacuum evaporation under reduced pressure
Extract drying temperature
35 °C
Analysis solvents
MeOH
Detection note
Columbamine is isomeric to jatrorrhizine. The relative intensities (%) of the fragments were, 327.1127 (100), 322.1073 (99), 294.1113 (77), 308.0908 (44), 280.0985 (23.3), 279.089 (20.1), 306.0762 (19.7), 265.0739 (10.6).
References

A. Singh, V. Bajpai, M. Srivastava, K. Arya, and B. Kumar, "Rapid profiling and structural characterization of bioactive compounds and their distribution in different parts of Berberis petiolaris Wall. ex G. Don applying hyphenated mass spectrometric techniques," Rapid Communications in Mass Spectrometry , vol. 28 , no. 19 , pp. 2089-2100 , DOI: 10.1002/rcm.7001 .