Chemical fact sheet: Ellagic acid

Ellagic acid

Basics

Category
Hydroxycinnamic & hydroxybenzoic acid derivatives & other organic acid derivatives
IUPAC-name
2,3,7,8-tetrahydroxychromeno[5,4,3-cde]chromene-5,10-dione
Formula
No formula stored
Exact mass
302.00630 g/mol
Molecular weight
No weights stored
Structure
Chemical structure of ellagic acid
Figure 1.1: Chemical structure of ellagic acid

Sources

In summary, the chemical ellagic acid has been analyzed from following sources:

Filipendula ulmaria  (L.) Maxim.
1st
Filipendula vulgaris  Moench
1st
Morus alba  L.
1st 2nd

Note that an analysis result in the database may indicate either presence or lack thereof of a chemical in an analyzed sample.

References

  1. M. Natić, D. Dabić, A. Papetti, M. Fotirić Akšić, V. Ognjanov, M. Ljubojević, and Ž. Tešić, "Analysis and characterisation of phytochemicals in mulberry (Morus alba L.) fruits grown in Vojvodina, North Serbia.," Food Chemistry , vol. 171 , pp. 128–136 , DOI: 10.1016/j.foodchem.2014.08.101 .
  2. S. Samardžić, J. Arsenijević, D. Božić, M. Milenković, V. Tešević, and Z. Maksimović, "Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.," Journal of Ethnopharmacology , vol. 213 , pp. 132–137 , DOI: 10.1016/j.jep.2017.11.013 .

Analysis results

Analysis result 1

Detection technique Values Units
[M⁻ H]⁻ 300.99800 m/z
MS²⁻ 185
229
257
284
301 		m/z
STD
True
TLC
False
UV/Vis detector description
UHPLC-DAD
Mass spectrometer description
UHPLC-DAD-MS/MS, triple-quadrupole, LTQ (linear trap quadrupole), high resolution mass spectrometer (UHPLC OrbiTrap MS), heated electrospray ionization (HESI)
Organism
Morus alba  L.
cultivated
homogenized, frozen
Collection dates
2011-6
Sample note
The black coloured fruits from one genotype from the location Palanka, North Serbia were collected by the researchers. Each genotype as represented by one tree, and each sample was taken from one individual plant. The tree was over 30 years old and originated from seed. All berries were picked at the biologically ripe stage. The berries were picked cardinally-oriented branches with different directions arond the canopy. Harvest time was between 10 and 20th June 2011. After picking, the fruits were stored at -20C until chemical analysis.
Dried material storage temperature
-20 °C
Extraction solvents
methanol containing 0.1% HCl
Extraction mass/volume-ratio
25 mg/mL
Extraction repeats
4
Extraction time
4 d 4 h
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Analysis solvents
MeOH:water (60:40)
Detection note
MS2 fragments (% base peak): 257 (100), 229 (70), 301 (50), 185 (40), 284 (30)
References

M. Natić, D. Dabić, A. Papetti, M. Fotirić Akšić, V. Ognjanov, M. Ljubojević, and Ž. Tešić, "Analysis and characterisation of phytochemicals in mulberry (Morus alba L.) fruits grown in Vojvodina, North Serbia.," Food Chemistry , vol. 171 , pp. 128–136 , DOI: 10.1016/j.foodchem.2014.08.101 .

Analysis result 2

Detection technique Values Units
[M⁻ H]⁻ 300.99800 m/z
MS²⁻ 185
229
257
284
301 		m/z
STD
True
TLC
False
UV/Vis detector description
UHPLC-DAD
Mass spectrometer description
UHPLC-DAD-MS/MS, triple-quadrupole, LTQ (linear trap quadrupole), high resolution mass spectrometer (UHPLC OrbiTrap MS), heated electrospray ionization (HESI)
Organism
Morus alba  L.
cultivated
homogenized, frozen
Collection dates
2011-6
Sample note
The black coloured fruits from one genotype from the location Novi Sad, North Serbia were collected by the researchers. Each genotype as represented by one tree, and each sample was taken from one individual plant. The tree was over 30 years old and originated from seed. All berries were picked at the biologically ripe stage. The berries were picked cardinally-oriented branches with different directions arond the canopy. Harvest time was between 10 and 20th June 2011. After picking, the fruits were stored at -20C until chemical analysis.
Dried material storage temperature
-20 °C
Extraction solvents
methanol containing 0.1% HCl
Extraction mass/volume-ratio
25 mg/mL
Extraction repeats
4
Extraction time
4 d 4 h
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Analysis solvents
MeOH:water (60:40)
Detection note
MS2 fragments (% base peak): 257 (100), 229 (70), 301 (50), 185 (40), 284 (30)
References

M. Natić, D. Dabić, A. Papetti, M. Fotirić Akšić, V. Ognjanov, M. Ljubojević, and Ž. Tešić, "Analysis and characterisation of phytochemicals in mulberry (Morus alba L.) fruits grown in Vojvodina, North Serbia.," Food Chemistry , vol. 171 , pp. 128–136 , DOI: 10.1016/j.foodchem.2014.08.101 .

Analysis result 3

Detection technique Values Units
UV/Vis ND nm
STD
True
TLC
False
UV/Vis detector description
HPLC-DAD, HPLC-PDA
Mass spectrometer description
LC-ESI-MS
Organism
Filipendula ulmaria  (L.) Maxim.
wild
ground, dried
Collection dates
2016-7
Sample note
Plant material was identified by Professor Branislava Lakušik (Department of Botany, University of Belgrade - Faculty of Pharmacy) and voucher specimen; number 3872HFF was deposited in the Herbarium of the Department of Botany, University of Belgrade - Faculty of Pharmacy.
Extraction solvents
boiling water
Extraction repeats
1
Extraction time
30 min
Extract drying method
freeze-drying
Analysis solvents
aqueous infusion
Detection note
The UV spectral data was not shown.
References

S. Samardžić, J. Arsenijević, D. Božić, M. Milenković, V. Tešević, and Z. Maksimović, "Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.," Journal of Ethnopharmacology , vol. 213 , pp. 132–137 , DOI: 10.1016/j.jep.2017.11.013 .

Analysis result 4

STD
True
TLC
False
UV/Vis detector description
HPLC-DAD, HPLC-PDA
Mass spectrometer description
LC-ESI-MS
Organism
Filipendula vulgaris  Moench
wild
ground, dried
Collection dates
2013-5, 2014-5
Sample note
Plant material was collected in 2013 and 2014 (analysed together) near Loćika and identified by Professor Branislava Lakušik (Department of Botany, University of Belgrade - Faculty of Pharmacy) and voucher specimen; number 3713HFF was deposited in the Herbarium of the Department of Botany, University of Belgrade - Faculty of Pharmacy.
Extraction solvents
boiling water
Extraction repeats
1
Extraction time
30 min
Extract drying method
freeze-drying
Analysis solvents
aqueous infusion
Detection note
The compound was quantified; the UV spectral data was not shown.
References

S. Samardžić, J. Arsenijević, D. Božić, M. Milenković, V. Tešević, and Z. Maksimović, "Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.," Journal of Ethnopharmacology , vol. 213 , pp. 132–137 , DOI: 10.1016/j.jep.2017.11.013 .