Chemical fact sheet: Indoxyl

Indoxyl

Basics

Category
Indolics & indole alkaloid derivatives
IUPAC-name
1H-indol-3-ol
Formula
No formula stored
Exact mass
133.05280 g/mol
Molecular weight
No weights stored
Structure
Chemical structure of indoxyl
Figure 1.1: Chemical structure of indoxyl

Sources

In summary, the chemical indoxyl has been analyzed from following sources:

Isatis tinctoria 'IT BM'  L.
1st
Isatis tinctoria Thüringer Waid  L.
1st

Note that an analysis result in the database may indicate either presence or lack thereof of a chemical in an analyzed sample.

References

  1. T. Maugard, E. Enaud, P. Choisy, and M. Legoy, "Identification of an indigo precursor from leaves of Isatis tinctoria (woad).," Phytochemistry , vol. 58 , no. 6 , pp. 897–904 , DOI: 10.1016/S0031-9422(01)00335-1 .
  2. M. Tobias, I. Plitzko, and M. Hamburger, "A comprehensive metabolite profiling of Isatis tinctoria leaf extracts.," Phytochemistry , vol. 70 , no. 7 , pp. 924-934 , DOI: 10.1016/j.phytochem.2009.04.019 .

Analysis results

Analysis result 1

Detection technique Values Units
UV/Vis 240 nm
[M⁺ H]⁺ 134 m/z
STD
False
TLC
False
UV/Vis detector description
HPLC-DAD, UV
Mass spectrometer description
HPLC-MS
Organism
Isatis tinctoria 'IT BM'  L.
cultivated
fresh
Collection dates
2000-6
Sample note
The cultivar IT BM was grown in a field of CRITT (St Savin, France) and was collected by the researchers.
Extraction solvents
acetone:acetic acid 1% (v/v)
Extraction mass/volume-ratio
100 mg/mL
Extraction time
4 h
Extraction temperature
24 °C
Extract drying method
evaporation under reduced pressure
Analysis solvents
acetic acid (was left in the extract); MeOH
References

T. Maugard, E. Enaud, P. Choisy, and M. Legoy, "Identification of an indigo precursor from leaves of Isatis tinctoria (woad).," Phytochemistry , vol. 58 , no. 6 , pp. 897–904 , DOI: 10.1016/S0031-9422(01)00335-1 .

Analysis result 2

Detection technique Values Units
UV/Vis 205
248 		nm
[M⁺ H]⁺ 134 m/z
MS²⁺ 114 m/z
STD
True
TLC
False
UV/Vis detector description
HPLC-PDA, photodiode array (PDA)
Mass spectrometer description
HPLC-ESI-MS, ion trap, HPLC-TOF-MS, APCI
Organism
Isatis tinctoria Thüringer Waid  L.
cultivated
ground, dried, cut into small pieces
Collection dates
2003-9
Sample note
The plants were cultivated on the experimental plots of the Agricultural Research Station of Thuringia (TLL), Dornburg, Germany. Specimens of seeds from the strains are kept at the TLL, under accessions number 153/PG 1 (Thüringer Waid).
Drying methods
oven-dried
Drying temperature
40 °C
Dried material storage temperature
15 °C
Dried material storage notes
dark; in the brown glass bottles
Extraction solvents
dichloromethane
Extraction repeats
2
Extraction time
12 min
Extraction temperature
70 °C
Analysis solvents
dichloromethane
Detection note
[M + H]+ 134 (both ESI+ and APCI +)
References

M. Tobias, I. Plitzko, and M. Hamburger, "A comprehensive metabolite profiling of Isatis tinctoria leaf extracts.," Phytochemistry , vol. 70 , no. 7 , pp. 924-934 , DOI: 10.1016/j.phytochem.2009.04.019 .