Analysis result 1
UV/Vis detector description
HPLC-PDA
Mass spectrometer description
Organism
Tanacetum vulgare
L.
Sample note
Plant raw materials, herbs of T. vulgare, at full flowering state; were obtained from the Botanical Garden of Medicinal and Aromatic Plants (Koryciny, Poland). Voucher specimen were deposited at herbarium of Warsaw Universit of Life Sciences, WULS-SGGW, Poland.
Drying methods
air-dried, dark
Extraction solvents
ethanol:water, 40:60, v/v
Extraction mass/volume-ratio
100 mg/mL
Extraction time
5 h 10 min
Extract liquid storage temperature
-80 °C
Extract drying method
concentration under vacuum
Dried extract storage temperature
4 °C
References
K.
Bączek,
O.
Kosakowska,
J.
Przybył,
E.
Pióro-Jabrucka,
R.
Costa,
L.
Mondello,
M.
Gniewosz,
A.
Synowiec,
and
Z.
Węglarz,
"Antibacterial and antioxidant activity of essential oils and extracts from costmary (Tanacetum balsamita L.) and tansy (Tanacetum vulgare L.).,"
Industrial Crops and Products
,
vol. 102
,
pp. 154–163
,
DOI: 10.1016/j.indcrop.2017.03.009
.
Analysis result 2
| Detection technique |
Values |
Units |
| UV/Vis |
290
sh
325
|
nm |
| [M⁻ H]⁻ |
193
|
m/z |
| MS²⁻ |
133
134
161
|
m/z |
UV/Vis detector description
PDA, UHPLC-photodiode array detector
Mass spectrometer description
ESI, UPLC-PDA-ESI-MS/MS, tandem quadrupole mass spectrometer, TQD
Organism
Taraxacum officinale
G.H. Weber ex F. H. Wigg.
Sample note
The dandelion (Taraxacum officinale L.) root (Taraxaci radix) was identified by Prof. Krzysztof Oklejewicz (Department of Botany, University of Rzeszów, Poland). A voucher specimen has been deposited at the Department of Biochemistry and Crop Quality of the Institute of Soil Science and Plant Cultivation - State Research Institute in Pulawy.
Drying methods
freeze-dried
Dried material storage notes
dark; in a refrigerator; as pulverized
Extraction solvents
80 % methanol
Extraction mass/volume-ratio
46.7 mg/mL
Extraction temperature
20±5 °C
Extract drying method
evaporation under vacuum
Extract drying temperature
40 °C
Analysis solvents
50 % MeOH
References
D.
Jedrejek,
B.
Lis,
A.
Rolnik,
A.
Stochmal,
and
B.
Olas,
"Comparative phytochemical, cytotoxicity, antioxidant and haemostatic studies of Taraxacum officinale root preparations.,"
Food and Chemical Toxicology
,
vol. 126
,
pp. 233–247
,
DOI: 10.1016/j.fct.2019.02.017
.
Analysis result 3
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 4
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 5
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 6
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 7
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were yellow and the pistils became orange as the development proceeded, and the colour started to change gradually yellos/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 8
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791)) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to yellow&/red. The pistils became orange as the development proceeded.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 9
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
193.05000
|
m/z |
| MS²⁻ |
134
149
178
|
m/z |
UV/Vis detector description
UHPLC-DAD
Mass spectrometer description
UHPLC-DAD-MS/MS, triple-quadrupole, LTQ (linear trap quadrupole), high resolution mass spectrometer (UHPLC OrbiTrap MS), heated electrospray ionization (HESI)
Sample note
The black coloured fruits from one genotype from the location Palanka, North Serbia were collected by the researchers. Each genotype as represented by one tree, and each sample was taken from one individual plant. The tree was over 30 years old and originated from seed. All berries were picked at the biologically ripe stage. The berries were picked cardinally-oriented branches with different directions arond the canopy. Harvest time was between 10 and 20th June 2011. After picking, the fruits were stored at -20C until chemical analysis.
Dried material storage temperature
-20 °C
Extraction solvents
methanol containing 0.1% HCl
Extraction mass/volume-ratio
25 mg/mL
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Analysis solvents
MeOH:water (60:40)
Detection note
MS2 fragments (% base peak): 134 (100), 149 (40), 178 (30)
References
M.
Natić,
D.
Dabić,
A.
Papetti,
M.
Fotirić Akšić,
V.
Ognjanov,
M.
Ljubojević,
and
Ž.
Tešić,
"Analysis and characterisation of phytochemicals in mulberry (Morus alba L.) fruits grown in Vojvodina, North Serbia.,"
Food Chemistry
,
vol. 171
,
pp. 128–136
,
DOI: 10.1016/j.foodchem.2014.08.101
.
Analysis result 10
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
193.05000
|
m/z |
| MS²⁻ |
134
149
178
|
m/z |
UV/Vis detector description
UHPLC-DAD
Mass spectrometer description
UHPLC-DAD-MS/MS, triple-quadrupole, LTQ (linear trap quadrupole), high resolution mass spectrometer (UHPLC OrbiTrap MS), heated electrospray ionization (HESI)
Sample note
The black coloured fruits from one genotype from the location Novi Sad, North Serbia were collected by the researchers. Each genotype as represented by one tree, and each sample was taken from one individual plant. The tree was over 30 years old and originated from seed. All berries were picked at the biologically ripe stage. The berries were picked cardinally-oriented branches with different directions arond the canopy. Harvest time was between 10 and 20th June 2011. After picking, the fruits were stored at -20C until chemical analysis.
Dried material storage temperature
-20 °C
Extraction solvents
methanol containing 0.1% HCl
Extraction mass/volume-ratio
25 mg/mL
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Analysis solvents
MeOH:water (60:40)
Detection note
MS2 fragments (% base peak): 134 (100), 149 (40), 178 (30)
References
M.
Natić,
D.
Dabić,
A.
Papetti,
M.
Fotirić Akšić,
V.
Ognjanov,
M.
Ljubojević,
and
Ž.
Tešić,
"Analysis and characterisation of phytochemicals in mulberry (Morus alba L.) fruits grown in Vojvodina, North Serbia.,"
Food Chemistry
,
vol. 171
,
pp. 128–136
,
DOI: 10.1016/j.foodchem.2014.08.101
.
Analysis result 11
| Detection technique |
Values |
Units |
| UV/Vis |
233
300
sh
321
|
nm |
| [M⁻ H]⁻ |
193
|
m/z |
UV/Vis detector description
HPLC-DAD, diode array detector
Mass spectrometer description
HPLC-ESI-MS, HPLC-DAD-ESI-MS
Organism
Taraxacum mongolicum
Hand.-Mazz.
Sample note
The aerial part of Taraxacum mongolicum Hand.-Mazz. was identified by Prof. Liurong Chen. The voucher specimen (TM20041-02) was deposited in Department of Traditional Chinese Medicine and Natural Drug Research, College of Pharmaceutical Sciences, Zheijiang University.
Extraction solvents
methanol
Extraction mass/volume-ratio
100 mg/mL
Extraction time
4 h 30 min
Extract drying method
concentration under reduced pressure
Extract drying temperature
45 °C
Dried extract storage temperature
4 °C
References
S.
Shi,
Y.
Zhao,
H.
Zhou,
Y.
Zhang,
X.
Jiang,
and
K.
Huang,
"Identification of antioxidants from Taraxacum mongolicum by high-performance liquid chromatography–diode array detection–radical scavenging detection–electrospray ionization mass spectrometry and nuclear magnetic resonance experiments.,"
Journal of Chromatography A
,
vol. 1209
,
pp. 145–152
,
DOI: 10.1016/j.chroma.2008.09.004
.
Analysis result 12
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
193
|
m/z |
| MS²⁻ |
134
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Dimitrograd was no. 2-1765. The aerial parts were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z) are presented, respectively, from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 13
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
193
|
m/z |
| MS²⁻ |
134
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Dimitrograd was no. 2-1765. The bulbs were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z) are presented, respectively, from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 14
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
193
|
m/z |
| MS²⁻ |
134
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Babusnica was no. 2-1767. The aerial parts were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z) are presented, respectively, from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 15
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
193
|
m/z |
| MS²⁻ |
134
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Babusnica was no. 2-1767. The bulbs were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z) are presented, respectively, from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
