Analysis result 1
| Detection technique |
Values |
Units |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
Mass spectrometer description
HPLC-DAD-ESI-MS/MS
Organism
Sambucus canadensis
'Johns'
Collection dates
2004-7, 2004-8
Sample note
The fruits were harvesed from early July to mid August in 2004 from plants grown at the US Department of Agriculture Agricultural Research Service (USDA-ARS) National Clonal Germplasm Repository in Corvallis, OR, USA. Cultivar Johns is originated from wild selection from Ontario released in NOva Scotia, 1954.
Dried material storage temperature
-20 °C
Extraction solvents
acidified methanol (0.1 % v/v formic acid)
Extraction mass/volume-ratio
167 mg/mL
Extract liquid storage temperature
-80 °C
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Analysis solvents
distilled water
References
J.
Lee,
and
C.
Finn,
"Anthocyanins and other polyphenolics in American elderberry (Sambucus canadensis) and European elderberry (S. nigra) cultivars.,"
Journal of the Science of Food and Agriculture
,
vol. 87
,
no. 14
,
pp. 2665-2675
,
DOI: 10.1002/jsfa.3029
.
Analysis result 2
| Detection technique |
Values |
Units |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
Mass spectrometer description
HPLC-DAD-ESI-MS/MS
Organism
Sambucus canadensis
'Johns'
Sample note
The fruits were harvested in August in 2005 from plants grown at the US Department of Agriculture Agricultural Research Service (USDA-ARS) National Clonal Germplasm Repository in Corvallis, OR, USA. Cultivar Johns is originated from wild selection from Ontario released in NOva Scotia, 1954.
Dried material storage temperature
-20 °C
Extraction solvents
acidified methanol (0.1 % v/v formic acid)
Extraction mass/volume-ratio
167 mg/mL
Extract liquid storage temperature
-80 °C
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Analysis solvents
distilled water
References
J.
Lee,
and
C.
Finn,
"Anthocyanins and other polyphenolics in American elderberry (Sambucus canadensis) and European elderberry (S. nigra) cultivars.,"
Journal of the Science of Food and Agriculture
,
vol. 87
,
no. 14
,
pp. 2665-2675
,
DOI: 10.1002/jsfa.3029
.
Analysis result 3
| Detection technique |
Values |
Units |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
Mass spectrometer description
HPLC-DAD-ESI-MS/MS
Organism
Sambucus canadensis
'York'
Collection dates
2004-7, 2004-8
Sample note
The fruits were harvesed from early July to mid August in 2004 from plants grown at the US Department of Agriculture Agricultural Research Service (USDA-ARS) National Clonal Germplasm Repository in Corvallis, OR, USA. Cultivar York is a hybrid between cultivars Adams 2 and Ezyoff, released in New York, in 1964.
Dried material storage temperature
-20 °C
Extraction solvents
acidified methanol (0.1 % v/v formic acid)
Extraction mass/volume-ratio
167 mg/mL
Extract liquid storage temperature
-80 °C
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Analysis solvents
distilled water
References
J.
Lee,
and
C.
Finn,
"Anthocyanins and other polyphenolics in American elderberry (Sambucus canadensis) and European elderberry (S. nigra) cultivars.,"
Journal of the Science of Food and Agriculture
,
vol. 87
,
no. 14
,
pp. 2665-2675
,
DOI: 10.1002/jsfa.3029
.
Analysis result 4
| Detection technique |
Values |
Units |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
Mass spectrometer description
HPLC-DAD-ESI-MS/MS
Organism
Sambucus canadensis
'York'
Sample note
The fruits were harvested in August in 2005 from plants grown at the US Department of Agriculture Agricultural Research Service (USDA-ARS) National Clonal Germplasm Repository in Corvallis, OR, USA. Cultivar Johns is originated from wild selection from Ontario released in NOva Scotia, 1954.
Dried material storage temperature
-20 °C
Extraction solvents
acidified methanol (0.1 % v/v formic acid)
Extraction mass/volume-ratio
167 mg/mL
Extract liquid storage temperature
-80 °C
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Analysis solvents
distilled water
References
J.
Lee,
and
C.
Finn,
"Anthocyanins and other polyphenolics in American elderberry (Sambucus canadensis) and European elderberry (S. nigra) cultivars.,"
Journal of the Science of Food and Agriculture
,
vol. 87
,
no. 14
,
pp. 2665-2675
,
DOI: 10.1002/jsfa.3029
.
Analysis result 5
| Detection technique |
Values |
Units |
| UV/Vis |
520
|
nm |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
photodiode-array (PDA), HPLC-UV/Vis-MS
Mass spectrometer description
ESI-MS
Organism
Aronia melanocarpa
(Michx.) Elliott.
Sample note
The researchers collected the fruits of black chokeberries.
Extraction solvents
0.1 % HCl in methanol
Extraction mass/volume-ratio
50 mg/mL
Extract drying method
concentrated in vacuo to a small volume (about 5L)
Analysis solvents
0.1 % HCl in MeOH
References
R.
Slimestad,
K.
Torskangerpoll,
H.
Nateland,
T.
Johannessen,
and
N.
Giske,
"Flavonoids from black chokeberries, Aronia melanocarpa.,"
Journal of Food Composition and Analysis
,
vol. 18
,
no. 1
,
pp. 61–68
,
DOI: 10.1016/j.jfca.2003.12.003
.
Analysis result 6
| Detection technique |
Values |
Units |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
HPLC-DAD
Mass spectrometer description
ion-trap, HPLC-DAD-ESI-MS/MS
Organism
Ribes nigrum
'Ben Alder'
Sample note
The black currants (cultivar Ben Alder) were obtained from Wisley Gardens, Royal Horticultural Society, England.Fresh berry samples were frozen and stored at -20 C until transferred to the Arkansas Children's Nutrition Center, at which time the frozen samples were freeze-dried and ground into powder. The powder was kept at -70 C until analysed.
Drying methods
freeze-dried
Dried material storage temperature
-70 °C
Extraction solvents
MeOH:water:acetic acid (85:15:0,5, v/v)
Extraction mass/volume-ratio
40 mg/mL
Analysis solvents
methanol; water; acetic acid
References
X.
Wu,
L.
Gu,
R.
Prior,
and
S.
McKay,
"Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity,"
Journal of Agricultural and Food Chemistry
,
vol. 52
,
no. 26
,
pp. 7846–7856
,
DOI: 10.1021/jf0486850
.
Analysis result 7
| Detection technique |
Values |
Units |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
HPLC-DAD
Mass spectrometer description
ion-trap, HPLC-DAD-ESI-MS/MS
Organism
Ribes nigrum
'Ben Nevis'
Sample note
The black currants (cultivar Ben Nevis) were obtained from Wisley Gardens, Royal Horticultural Society, England.Fresh berry samples were frozen and stored at -20 C until transferred to the Arkansas Children's Nutrition Center, at which time the frozen samples were freeze-dried and ground into powder. The powder was kept at -70 C until analysed.
Drying methods
freeze-dried
Dried material storage temperature
-70 °C
Extraction solvents
MeOH:water:acetic acid (85:15:0,5, v/v)
Extraction mass/volume-ratio
40 mg/mL
Analysis solvents
methanol; water; acetic acid
References
X.
Wu,
L.
Gu,
R.
Prior,
and
S.
McKay,
"Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity,"
Journal of Agricultural and Food Chemistry
,
vol. 52
,
no. 26
,
pp. 7846–7856
,
DOI: 10.1021/jf0486850
.
Analysis result 8
| Detection technique |
Values |
Units |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
HPLC-DAD
Mass spectrometer description
ion-trap, HPLC-DAD-ESI-MS/MS
Organism
Ribes nigrum
'Ben Lomond'
Sample note
The black currants (cultivar Ben Lomond) were obtained from Wisley Gardens, Royal Horticultural Society, England.Fresh berry samples were frozen and stored at -20 C until transferred to the Arkansas Children's Nutrition Center, at which time the frozen samples were freeze-dried and ground into powder. The powder was kept at -70 C until analysed.
Drying methods
freeze-dried
Dried material storage temperature
-70 °C
Extraction solvents
MeOH:water:acetic acid (85:15:0,5, v/v)
Extraction mass/volume-ratio
40 mg/mL
Analysis solvents
methanol; water; acetic acid
References
X.
Wu,
L.
Gu,
R.
Prior,
and
S.
McKay,
"Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity,"
Journal of Agricultural and Food Chemistry
,
vol. 52
,
no. 26
,
pp. 7846–7856
,
DOI: 10.1021/jf0486850
.
Analysis result 9
| Detection technique |
Values |
Units |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
HPLC-DAD
Mass spectrometer description
ion-trap, HPLC-DAD-ESI-MS/MS
Organism
Ribes nigrum
'Titania'
Sample note
The black currants (cultivar Titania) were obtained from Wisley Gardens, Royal Horticultural Society, England.Fresh berry samples were frozen and stored at -20 C until transferred to the Arkansas Children's Nutrition Center, at which time the frozen samples were freeze-dried and ground into powder. The powder was kept at -70 C until analysed.
Drying methods
freeze-dried
Dried material storage temperature
-70 °C
Extraction solvents
MeOH:water:acetic acid (85:15:0,5, v/v)
Extraction mass/volume-ratio
40 mg/mL
Analysis solvents
methanol; water; acetic acid
References
X.
Wu,
L.
Gu,
R.
Prior,
and
S.
McKay,
"Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity,"
Journal of Agricultural and Food Chemistry
,
vol. 52
,
no. 26
,
pp. 7846–7856
,
DOI: 10.1021/jf0486850
.
Analysis result 10
| Detection technique |
Values |
Units |
| M⁺ |
449
|
m/z |
| MS²⁺ |
287
|
m/z |
UV/Vis detector description
HPLC-DAD
Mass spectrometer description
ion-trap, HPLC-DAD-ESI-MS/MS
Organism
Ribes nigrum
'Ben Tirran'
Sample note
The black currants (cultivar Ben Tirran) were obtained from Wisley Gardens, Royal Horticultural Society, England.Fresh berry samples were frozen and stored at -20 C until transferred to the Arkansas Children's Nutrition Center, at which time the frozen samples were freeze-dried and ground into powder. The powder was kept at -70 C until analysed.
Drying methods
freeze-dried
Dried material storage temperature
-70 °C
Extraction solvents
MeOH:water:acetic acid (85:15:0,5, v/v)
Extraction mass/volume-ratio
40 mg/mL
Analysis solvents
methanol; water; acetic acid
References
X.
Wu,
L.
Gu,
R.
Prior,
and
S.
McKay,
"Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity,"
Journal of Agricultural and Food Chemistry
,
vol. 52
,
no. 26
,
pp. 7846–7856
,
DOI: 10.1021/jf0486850
.
