Chemical fact sheet: Kaempferol 3-O-β-D-glucoside-7-O-β-D-glucuronide

Kaempferol 3-O-β-D-glucoside-7-O-β-D-glucuronide

Basics

Category
Flavone and flavonol derivatives
IUPAC-name
(2S,3S,4S,5R)-3,4,5-trihydroxy-6-((5-hydroxy-2-(4-hydroxyphenyl)-4-oxo-3-(((3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-4H-chromen-7-yl)oxy)tetrahydro-2H-pyran-2-carboxylic acid
Formula
No formula stored
Exact mass
624.13265 g/mol
Molecular weight
No weights stored
Structure
Chemical structure of kaempferol 3-O-β-D-glucoside-7-O-β-D-glucuronide
Figure 1.1: Chemical structure of kaempferol 3-O-β-D-glucoside-7-O-β-D-glucuronide

Sources

In summary, the chemical kaempferol 3-O-β-D-glucoside-7-O-β-D-glucuronide has been analyzed from following sources:

Carthamus tinctorius 'Ken-ba'
1st
Carthamus tinctorius 'Ogon-hanagasa'
1st
Carthamus tinctorius 'Shiro-bana'
1st

Note that an analysis result in the database may indicate either presence or lack thereof of a chemical in an analyzed sample.

References

  1. K. Kazuma, T. Takahashi, K. Sato, H. Takeuchi, T. Matsumoto, and T. Okuno, "Quinochalcones and flavonoids from fresh florets in different cultivars of Carthamus tinctorius L.," Bioscience, Biotechnology, and Biochemistry , vol. 64 , no. 8 , pp. 1588–1599 , DOI: 10.1271/bbb.64.1588 .

Analysis results

Analysis result 1

Detection technique Values Units
UV/Vis 266
349 		nm
[M⁺ H]⁺ 625 m/z
MS²⁺ 286.50000
462.50000 		m/z
STD
False
TLC
False
UV/Vis detector description
HPLC-PDA
Mass spectrometer description
ESI-MS/MS
Organism
Carthamus tinctorius 'Ken-ba'
fresh
Sample note
Cultivar Ken-ba was grown in the field of Hirosaki University in Japan. The capitula were harvested when tubular florets had just appeared outside the involucres. Only tubular florets were collected. Ken-ba is a wild type cultivar the petals of which look orange yellow. For flavonoid analysis, florets (3g9 were hoogenized and extracted with cold 1 % TFA-MeOH (2 mL x 3).
Extraction solvents
1 % TFA-MeOH
Extraction mass/volume-ratio
500 mg/mL
Extraction repeats
3
Extraction temperature
4 °C
Extract drying method
evaporation in vacuo
Analysis solvents
methanol:water (1:1, v7v)
Detection note
HR-FAB-MS m/z 647.1176 [M + Na]+ was found. White amorphous powder was obtained.
References

K. Kazuma, T. Takahashi, K. Sato, H. Takeuchi, T. Matsumoto, and T. Okuno, "Quinochalcones and flavonoids from fresh florets in different cultivars of Carthamus tinctorius L.," Bioscience, Biotechnology, and Biochemistry , vol. 64 , no. 8 , pp. 1588–1599 , DOI: 10.1271/bbb.64.1588 .

Analysis result 2

Detection technique Values Units
UV/Vis 266
349 		nm
[M⁺ H]⁺ 625 m/z
MS²⁺ 286.50000
462.50000 		m/z
STD
False
TLC
False
UV/Vis detector description
HPLC-PDA
Mass spectrometer description
ESI-MS/MS
Organism
Carthamus tinctorius 'Ogon-hanagasa'
fresh
Sample note
Cultivar Ogon-hanagasa was grown in the field of Hirosaki University in Japan. The capitula were harvested when tubular florets had just appeared outside the involucres. Only tubular florets were collected. Ken-ba is a wild type cultivar the petals of which look orange yellow. For flavonoid analysis, florets (3g9 were hoogenized and extracted with cold 1 % TFA-MeOH (2 mL x 3).
Extraction solvents
1 % TFA-MeOH
Extraction mass/volume-ratio
500 mg/mL
Extraction repeats
3
Extraction temperature
4 °C
Extract drying method
evaporation in vacuo
Analysis solvents
methanol:water (1:1, v7v)
Detection note
HR-FAB-MS m/z 647.1176 [M + Na]+ was found. White amorphous powder was obtained.
References

K. Kazuma, T. Takahashi, K. Sato, H. Takeuchi, T. Matsumoto, and T. Okuno, "Quinochalcones and flavonoids from fresh florets in different cultivars of Carthamus tinctorius L.," Bioscience, Biotechnology, and Biochemistry , vol. 64 , no. 8 , pp. 1588–1599 , DOI: 10.1271/bbb.64.1588 .

Analysis result 3

Detection technique Values Units
UV/Vis 266
349 		nm
[M⁺ H]⁺ 625 m/z
MS²⁺ 286.50000
462.50000 		m/z
STD
False
TLC
False
UV/Vis detector description
HPLC-PDA
Mass spectrometer description
ESI-MS/MS
Organism
Carthamus tinctorius 'Shiro-bana'
fresh
Sample note
Cultivar Shiro-bana was grown in the field of Hirosaki University in Japan. The capitula were harvested when tubular florets had just appeared outside the involucres. Only tubular florets were collected. Ken-ba is a wild type cultivar the petals of which look orange yellow. For flavonoid analysis, florets (3g9 were hoogenized and extracted with cold 1 % TFA-MeOH (2 mL x 3).
Extraction solvents
1 % TFA-MeOH
Extraction mass/volume-ratio
500 mg/mL
Extraction repeats
3
Extraction temperature
4 °C
Extract drying method
evaporation in vacuo
Analysis solvents
methanol:water (1:1, v7v)
Detection note
HR-FAB-MS m/z 647.1176 [M + Na]+ was found. White amorphous powder was obtained.
References

K. Kazuma, T. Takahashi, K. Sato, H. Takeuchi, T. Matsumoto, and T. Okuno, "Quinochalcones and flavonoids from fresh florets in different cultivars of Carthamus tinctorius L.," Bioscience, Biotechnology, and Biochemistry , vol. 64 , no. 8 , pp. 1588–1599 , DOI: 10.1271/bbb.64.1588 .