Analysis result 1
| Detection technique |
Values |
Units |
| UV/Vis |
230
410
|
nm |
| [M⁺ H]⁺ |
1045.28200
1045.50000
|
m/z |
| MS²⁺ |
433
721.50000
888.50000
|
m/z |
UV/Vis detector description
HPLC-PDA
Mass spectrometer description
ESI-MS/MS
Organism
Carthamus tinctorius
'Ken-ba'
Sample note
Cultivar Ken-ba was grown in the field of Hirosaki University in Japan. The capitula were harvested when tubular florets had just appeared outside the involucres. Only tubular florets were collected. Ken-ba is a wild type cultivar the petals of which look orange yellow.
Extraction solvents
methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
4 °C
Extract drying method
evaporation in vacuo
Analysis solvents
methanol:water (1:1, v7v)
Detection note
M/z 1045.2820 [M + H]+ was detected by HR-FAB-MS and m/z 1045.5 was detected by ESI-MS. Yellow amorphous powder was obtained.
References
K.
Kazuma,
T.
Takahashi,
K.
Sato,
H.
Takeuchi,
T.
Matsumoto,
and
T.
Okuno,
"Quinochalcones and flavonoids from fresh florets in different cultivars of Carthamus tinctorius L.,"
Bioscience, Biotechnology, and Biochemistry
,
vol. 64
,
no. 8
,
pp. 1588–1599
,
DOI: 10.1271/bbb.64.1588
.
Analysis result 2
| Detection technique |
Values |
Units |
| UV/Vis |
230
410
|
nm |
| [M⁺ H]⁺ |
1045.28200
1045.50000
|
m/z |
| MS²⁺ |
433
721.50000
888.50000
|
m/z |
UV/Vis detector description
HPLC-PDA
Mass spectrometer description
ESI-MS/MS
Organism
Carthamus tinctorius
'Ogon-hanagasa'
Sample note
Cultivar Ogon-hanagasa was grown in the field of Hirosaki University in Japan. The capitula were harvested when tubular florets had just appeared outside the involucres. Only tubular florets were collected. Ogon-hanagasa is a cultivar with plain yellow petals.
Extraction solvents
methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
4 °C
Extract drying method
evaporation in vacuo
Analysis solvents
methanol:water (1:1, v7v)
Detection note
M/z 1045.2820 [M + H]+ was detected by HR-FAB-MS and m/z 1045.5 was detected by ESI-MS. Yellow amorphous powder was obtained.
References
K.
Kazuma,
T.
Takahashi,
K.
Sato,
H.
Takeuchi,
T.
Matsumoto,
and
T.
Okuno,
"Quinochalcones and flavonoids from fresh florets in different cultivars of Carthamus tinctorius L.,"
Bioscience, Biotechnology, and Biochemistry
,
vol. 64
,
no. 8
,
pp. 1588–1599
,
DOI: 10.1271/bbb.64.1588
.
Analysis result 3
| Detection technique |
Values |
Units |
| UV/Vis |
230
410
|
nm |
| [M⁺ H]⁺ |
1045.28200
1045.50000
|
m/z |
| MS²⁺ |
433
721.50000
888.50000
|
m/z |
UV/Vis detector description
HPLC-PDA
Mass spectrometer description
ESI-MS/MS
Organism
Carthamus tinctorius
'Shiro-bana'
Sample note
Cultivar Shiro-bana was grown in the field of Hirosaki University in Japan. The capitula were harvested when tubular florets had just appeared outside the involucres. Only tubular florets were collected. Shiro-bana cultivar has white petals.
Extraction solvents
methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
4 °C
Extract drying method
evaporation in vacuo
Analysis solvents
methanol:water (1:1, v7v)
Detection note
M/z 1045.2820 [M + H]+ was detected by HR-FAB-MS and m/z 1045.5 was detected by ESI-MS. Yellow amorphous powder was obtained.
References
K.
Kazuma,
T.
Takahashi,
K.
Sato,
H.
Takeuchi,
T.
Matsumoto,
and
T.
Okuno,
"Quinochalcones and flavonoids from fresh florets in different cultivars of Carthamus tinctorius L.,"
Bioscience, Biotechnology, and Biochemistry
,
vol. 64
,
no. 8
,
pp. 1588–1599
,
DOI: 10.1271/bbb.64.1588
.
Analysis result 4
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 5
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 6
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 7
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 8
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 9
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 10
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791)) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to yellow&/red. The pistils became orange as the development proceeded.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
