Chemical fact sheet: Physcion

Physcion

Basics

Category
Anthraquinone and quinone derivatives
IUPAC-name
1,8-dihydroxy-3-methoxy-6-methylanthracene-9,10-dione
Formula
No formula stored
Exact mass
284.06847 g/mol
Molecular weight
284.26000 g/mol
Structure
Chemical structure of physcion
Figure 1.1: Chemical structure of physcion

Sources

In summary, the chemical physcion has been analyzed from following sources:

Rhamnus davurica  Pall.
1st
Rhamnus nipalensis  Laws
1st
Rhamnus saxatilis  Jacq.
1st
Rheum emodii
1st
Rheum officinale  Baill.
1st
Rheum palmatum  L.
1st

Note that an analysis result in the database may indicate either presence or lack thereof of a chemical in an analyzed sample.

References

  1. G. Chen, X. Li, F. Saleri, and M. Guo, "Analysis of flavonoids in Rhamnus davurica and its antiproliferative effects.," Molecules , vol. 21 , no. 10 , pp. 1275 , DOI: 10.3390/molecules21101275 .
  2. J. Liu, L. Leng, Y. Liu, H. Gao, W. Yang, S. Chen, and A. Liu, "Identification and quantification of target metabolites combined with transcriptome of two rheum species focused on anthraquinone and flavonoids biosynthesis.," Scientific Reports , vol. 10 , no. 1 , pp. 20241 , DOI: 10.1038/s41598-020-77356-9 .
  3. M. Locatelli, F. Tammaro, L. Menghini, G. Carlucci, F. Epifano, and S. Genovese, "Anthraquinone profile and chemical fingerprint of Rhamnus saxatilis L. from Italy.," Phytochemistry Letters , vol. 2 , pp. 223–226 , DOI: 10.1016/j.phytol.2009.10.001 .
  4. M. Pandey, A. Singh, U. Singh, S. Singh, and V. Pandey, "A new chalcone glycoside from Rhamnus nipalensis.," Natural Product Research , vol. 22 , no. 18 , pp. 1657–1659 , DOI: 10.1080/14786410701876635 .
  5. S. Verma, N. Singh, and A. Sinha, "Determination and locational variations in the quantity of hydroxyanthraquinones and their glycosides in rhizomes of Rheum emodi using high-performance liquid chromatography.," Journal of Chromatography A , vol. 1097 , pp. 59–65 , DOI: 10.1016/j.chroma.2005.08.018 .

Analysis results

Analysis result 1

Detection technique Values Units
[M⁻ H]⁻ 283 m/z
MS²⁻ 211
239
267
268
283 		m/z
STD
False
TLC
False
UV/Vis detector description
Mass spectrometer description
ESI-MS/MS
Organism
Rhamnus davurica  Pall.
dried, powdered
Sample note
The authentication and identification of the barks of Rhamnus davurica was performed with the researchers of this study which were assisted by the taxonomist Quangwan Hu from Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture (Wuhan Botanicl Garden), Chinese Academy of Sciences. A voucher specimen (No. 0031) was deposited in the herbarium of the Key Laboratory.
Drying temperature
40 °C
Dried material storage temperature
4 °C
Dried material storage notes
the samples were packed in sealed polyethylene bags; stored in a refrigerator until use; dark
Extraction solvents
60 % ethanol
Extraction repeats
3
Extraction time
1 h 30 min
Extraction temperature
20±5 °C
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
Analysis solvents
MeOH
References

G. Chen, X. Li, F. Saleri, and M. Guo, "Analysis of flavonoids in Rhamnus davurica and its antiproliferative effects.," Molecules , vol. 21 , no. 10 , pp. 1275 , DOI: 10.3390/molecules21101275 .

Analysis result 2

Detection technique Values Units
[M⁻ H]⁻ 283.30000 m/z
MS²⁻ 240.10000 m/z
STD
True
TLC
False
UV/Vis detector description
UV/Vis
Mass spectrometer description
UPLC-ESI-Q-TOF-MS/MS
Organism
Rheum officinale  Baill.
cultivated
fresh, powdered
Sample note
The identification of Rheum officinale from the collected rhizome specimens were conducted by Prof. Li Xiang at the Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, China.
Dried material storage temperature
-80 °C
Extraction solvents
methanol
Extraction repeats
1
Extraction temperature
4 °C
Analysis solvents
methanol
References

J. Liu, L. Leng, Y. Liu, H. Gao, W. Yang, S. Chen, and A. Liu, "Identification and quantification of target metabolites combined with transcriptome of two rheum species focused on anthraquinone and flavonoids biosynthesis.," Scientific Reports , vol. 10 , no. 1 , pp. 20241 , DOI: 10.1038/s41598-020-77356-9 .

Analysis result 3

Detection technique Values Units
[M⁻ H]⁻ 283.30000 m/z
MS²⁻ 240.10000 m/z
STD
True
TLC
False
UV/Vis detector description
UV/Vis
Mass spectrometer description
UPLC-ESI-Q-TOF-MS/MS
Organism
Rheum palmatum  L.
cultivated
fresh, powdered
Sample note
The identification of Rheum palmatum from the collected rhizome specimens were conducted by Prof. Li Xiang at the Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, China.
Dried material storage temperature
-80 °C
Extraction solvents
methanol
Extraction repeats
1
Extraction temperature
4 °C
Analysis solvents
methanol
References

J. Liu, L. Leng, Y. Liu, H. Gao, W. Yang, S. Chen, and A. Liu, "Identification and quantification of target metabolites combined with transcriptome of two rheum species focused on anthraquinone and flavonoids biosynthesis.," Scientific Reports , vol. 10 , no. 1 , pp. 20241 , DOI: 10.1038/s41598-020-77356-9 .

Analysis result 4

STD
True
TLC
False
UV/Vis detector description
photodiode array
Mass spectrometer description
Organism
Rhamnus saxatilis  Jacq.
wild
Collection dates
2008-10
Sample note
The samples were authenticated in-house by F. Tammaro (Faculty of Biotechnology, University of L'Aquila, L'Aquila, Italy). A voucher specimen (GS-2008-1) was stored at the herbarium of the Giardino dei Semplici ot the University "G. D'Annunzio" of Chieti-Pescara./Ultrasonication was used in the extraction and the extract was hydrolysed with 6N HCl.
Extraction solvents
methanol (100 %), hydrochloric acid (6N), ethyl acetate
Extraction mass/volume-ratio
100 mg/mL
Extraction repeats
1
Extraction time
1 h
Extraction temperature
20±5 °C
Extract drying method
evaporation under vacuum
Dried extract storage temperature
-20 °C
Analysis solvents
methanol
Detection note
The methanol (100%) extract was hydrolyzed and organic phases were separated with liqui-liquid extraction with ethyl acetate and dried under vacuum.
References

M. Locatelli, F. Tammaro, L. Menghini, G. Carlucci, F. Epifano, and S. Genovese, "Anthraquinone profile and chemical fingerprint of Rhamnus saxatilis L. from Italy.," Phytochemistry Letters , vol. 2 , pp. 223–226 , DOI: 10.1016/j.phytol.2009.10.001 .

Analysis result 5

STD
True
TLC
False
UV/Vis detector description
UV
Mass spectrometer description
EI-HRMS
Organism
Rhamnus nipalensis  Laws
wild
as whole, dried, all parts
Sample note
The authenticity of Rhamnus nipalensis was verified by Prof. NK Dubey of the Department of Botany, Banaras Hindu University, Varanasi. A specimen voucher of the plant is kept in the Department.
Extraction solvents
methanol
Extraction temperature
25 °C
Analysis solvents
MeOH
Detection note
The authors referred: Ram SN, Dwivedi SPD, Pandey VB & Shah AH. 1988. Constituents of Rhamnus triquerta. Fitoterapia, LIX, 78–79.
References

M. Pandey, A. Singh, U. Singh, S. Singh, and V. Pandey, "A new chalcone glycoside from Rhamnus nipalensis.," Natural Product Research , vol. 22 , no. 18 , pp. 1657–1659 , DOI: 10.1080/14786410701876635 .

Analysis result 6

Detection technique Values Units
UV/Vis 222
266
287
434 		nm
STD
True
TLC
False
UV/Vis detector description
HPLC, HPLC-UV/Vis, HPLC-PDA
Mass spectrometer description
Organism
Rheum emodii
wild
dried, powdered
Sample note
The researchers collected the rhizomes of R. emodii from western Himalaya, Himachal pradesh, from three locations, India. /The extractions were performed separately with methanol using an ultrasonicator. The same compounds were analysed from the samples of all three origins.
Drying methods
air-dried
Drying temperature
25±2 °C
Extraction solvents
methanol
Extraction mass/volume-ratio
50 mg/mL
Extraction repeats
3
Extraction time
30 min
Extract drying method
evaporation under reduced pressure
Analysis solvents
methanol
References

S. Verma, N. Singh, and A. Sinha, "Determination and locational variations in the quantity of hydroxyanthraquinones and their glycosides in rhizomes of Rheum emodi using high-performance liquid chromatography.," Journal of Chromatography A , vol. 1097 , pp. 59–65 , DOI: 10.1016/j.chroma.2005.08.018 .