Chemical fact sheet: Apigenin 6,8-di-C-glucoside

STD

False

TLC

False

UV/Vis detector description

LC-diode array (DAD), PDA

Mass spectrometer description

ESI-MS, ion trap

Organism

Daphne gnidium  L.

wild

Collection dates

2007-5, 2007-6

Sample note

The samples were identified and collected by the researchers.

Extraction solvents

water

Extraction time

1 h

Extraction temperature

100 °C

Analysis solvents

water: MeOH, 8:2

Detection note

533 [M–H–60)–

References

R. Marques, M. Sousa, M. Oliveira, and M. Melo, "Characterization of weld (Reseda luteola L.) and spurge flax (Daphne gnidium L.) by high-performance liquid chromatography–diode array detection–mass spectrometry in Arraiolos historical textiles.," Journal of Chromatography A , vol. 1216 , pp. 1395–1402 , DOI: 10.1016/j.chroma.2008.12.083 .

STD

False

TLC

False

UV/Vis detector description

HPLC-PDA, photodiode array (PDA)

Mass spectrometer description

HPLC-ESI-MS, ion trap, HPLC-TOF-MS, APCI

Organism

Isatis tinctoria Thüringer Waid  L.

cultivated

ground, dried, cut into small pieces

Collection dates

2003-9

Sample note

The plants were cultivated on the experimental plots of the Agricultural Research Station of Thuringia (TLL), Dornburg, Germany. Specimens of seeds from the strains are kept at the TLL, under accessions number 153/PG 1 (Thüringer Waid).

Drying methods

oven-dried

Drying temperature

40 °C

Dried material storage temperature

15 °C

Dried material storage notes

dark; in the brown glass bottles

Extraction solvents

methanol

Extraction repeats

2

Extraction time

12 min

Extraction temperature

70 °C

Analysis solvents

MeOH

Detection note

[M + Na]+ = 617

References

M. Tobias, I. Plitzko, and M. Hamburger, "A comprehensive metabolite profiling of Isatis tinctoria leaf extracts.," Phytochemistry , vol. 70 , no. 7 , pp. 924-934 , DOI: 10.1016/j.phytochem.2009.04.019 .

STD

False

TLC

False

UV/Vis detector description

HPLC-PDA, photodiode array (PDA)

Mass spectrometer description

HPLC-ESI-MS, ion trap, HPLC-TOF-MS, APCI

Organism

Isatis indigotina

cultivated

ground, dried, cut into small pieces

Collection dates

2003-9

Sample note

The plants were cultivated on the experimental plots of the Agricultural Research Station of Thuringia (TLL), Dornburg, Germany. Specimens of seeds from the strains are kept at the TLL, under accessions number 153 PG 12 (I. indigotina).NOTE. There are conflicting views on the taxonomy of I. tinctoria (European woad) and I. indigotica (Chinese woad). I. indigotina was described for the first time by Fortune in 1846, and initially considered as a distinct species. Afterward, some taxonomists have classified it as a variety of I. tinctoria. Nowadays, I. indigotina is considered as a synonym of I. tinctoria [e.g. The Plant List, IPNI (International Plant Name Index), Tropicos]. (Speranzo et al. 2020). In this database, we use the names similarly as the authors.

Drying methods

oven-dried

Drying temperature

40 °C

Dried material storage temperature

15 °C

Dried material storage notes

dark; in the brown glass bottles

Extraction solvents

methanol

Extraction repeats

2

Extraction time

12 min

Extraction temperature

70 °C

Analysis solvents

MeOH

Detection note

[M + Na]+ = 617

References

M. Tobias, I. Plitzko, and M. Hamburger, "A comprehensive metabolite profiling of Isatis tinctoria leaf extracts.," Phytochemistry , vol. 70 , no. 7 , pp. 924-934 , DOI: 10.1016/j.phytochem.2009.04.019 .

STD

False

TLC

False

UV/Vis detector description

Mass spectrometer description

ESI, LTQ orbitrap, LTQ linear ion trap

Organism

Isatis tinctoria French Woad  L.

cultivated

fresh, frozen, cut into small pieces, frozen in liquid nitrogen

Sample note

The seeds were provided by the botanical garden of Amiens (Jardin des Plantes), France, where specimens of seeds from the strain were kept under accession number 061003-3. The seeds were sown in soil and grown in a greenhouse at 16h/8h day/night regime, 24 C, 70% moisture. The samples ere taken from 11-week-old plants at the rosette stage.

Extraction solvents

methanol

Extraction repeats

2

Extraction time

10 min

Extraction temperature

70 °C

Extract drying method

evaporation in the speed vacuum

Analysis solvents

water: MeOH

References

T. Nguyen, A. Jamali, E. Grand, K. Morreel, P. Marcelo, E. Gontier, and R. Dauwe, "Phenylpropanoid profiling reveals a class of hydroxycinnamoyl glucaric acid conjugates in Isatis tinctoria leaves," Phytochemistry , vol. 144 , pp. 127–140 , DOI: 10.1016/j.phytochem.2017.09.007 .

STD

False

TLC

False

UV/Vis detector description

Mass spectrometer description

ESI, LTQ orbitrap, LTQ linear ion trap

Organism

Isatis tinctoria French Woad  L.

cultivated

fresh, frozen, frozen in liquid nitrogen

Sample note

The seeds of Isatis tinctoria L.(strain French Woa; accession number 061003-3) were provided by the botanical garden of Amiens (Jardin des Plantes), France. They were sown in soil and plantlets were grown in greenhouse under the following conditions: 24 °C, 70% moisture and photoperiod 16h. Rosette leaves were harvested from 11-week-old woad plants.

Extraction solvents

methanol

Extraction repeats

2

Extraction time

10 min

Extraction temperature

70 °C

Extract drying method

evaporation in the speed vacuum

Analysis solvents

water: MeOH

Detection note

See. Nguyen et al. 2017 (MS-fragments)

References

T. Nguyen, P. Marcelo, E. Gontier, and R. Dauwec, "Metabolic markers for the yield of lipophilic indole alkaloids in dried woad leaves (Isatis tinctoria L.).," Phytochemistry , vol. 163 , pp. 89-98 , DOI: 10.1016/j.phytochem.2019.04.006 .