Chemical fact sheet: Kaempferol 3-O-galactoside

STD

True

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus palaestinus  L.

wild

dried, frozen

Sample note

The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

Detection note

The exact mass was obtained.

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

True

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus palaestinus  L.

wild

dried, frozen

Sample note

The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

Detection note

The exact mass was obtained.

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

True

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus palaestinus  L.

wild

dried, frozen

Sample note

The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

Detection note

The exact mass was obtained.

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

True

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus lanatus  L.

wild

dried, frozen

Sample note

The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The leaf shape was lanceolate.

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

True

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus lanatus  L.

wild

dried, frozen

Sample note

The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were yellow and the pistils became orange as the development proceeded, and the colour started to change gradually yellos/red.

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

True

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus lanatus  L.

wild

dried, frozen

Sample note

The material (Accession Number W6 16791)) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to yellow&/red. The pistils became orange as the development proceeded.

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .