Chemical fact sheet: Salicylic acid

STD

True

TLC

False

UV/Vis detector description

LC-DAD

Mass spectrometer description

LC-DAD-ESI-MS-MS, LC-ESI-MS-MS, triple quadrupole

Organism

Filipendula ulmaria  (L.) Maxim.

wild

dried, powdered

Collection dates

2007-6

Sample note

Filipendula ulmaria L. Maxim plant samples; herb and flower (Filipendulae herba and flos) were collected and identification was performed in the Department of Pharmacognosy, Semmelweis University, where the voucher specimens is deposited.

Drying methods

air-dried

Extraction solvents

methanol

Extraction repeats

1

Extract drying method

evaporation under reduced pressure

Extract drying temperature

50 °C

Analysis solvents

70 % methanol

Detection note

Salicylic acid was detected also in free form.

References

B. Blazics, I. Papp, and Á. Kéry, "LC-MS qualitative analysis and simultaneous determination of six Filipendula salicylates with two standards.," Chromatographia , vol. 71 , pp. S61–S677 , DOI: 10.1365/s10337-010-1502-4 .

STD

False

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus tinctorius  L.

cultivated

dried, frozen

Sample note

The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

Detection note

The exact mass was obtained.

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

False

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus lanatus  L.

wild

dried, frozen

Sample note

The material (Accession Number W6 16791)) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to yellow&/red. The pistils became orange as the development proceeded.

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

True

TLC

False

UV/Vis detector description

HPLC-DAD, HPLC-PDA

Mass spectrometer description

LC-ESI-MS

Organism

Filipendula ulmaria  (L.) Maxim.

wild

ground, dried

Collection dates

2016-7

Sample note

Plant material was identified by Professor Branislava Lakušik (Department of Botany, University of Belgrade - Faculty of Pharmacy) and voucher specimen; number 3872HFF was deposited in the Herbarium of the Department of Botany, University of Belgrade - Faculty of Pharmacy.

Extraction solvents

boiling water

Extraction repeats

1

Extraction time

30 min

Extract drying method

freeze-drying

Analysis solvents

aqueous infusion

Detection note

The compound was quantified; the UV spectral data was not shown.

References

S. Samardžić, J. Arsenijević, D. Božić, M. Milenković, V. Tešević, and Z. Maksimović, "Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.," Journal of Ethnopharmacology , vol. 213 , pp. 132–137 , DOI: 10.1016/j.jep.2017.11.013 .

STD

True

TLC

False

UV/Vis detector description

HPLC-DAD, HPLC-PDA

Mass spectrometer description

LC-ESI-MS

Organism

Filipendula vulgaris  Moench

wild

ground, dried

Collection dates

2013-5, 2014-5

Sample note

Plant material was collected in 2013 and 2014 (analysed together) near Loćika and identified by Professor Branislava Lakušik (Department of Botany, University of Belgrade - Faculty of Pharmacy) and voucher specimen; number 3713HFF was deposited in the Herbarium of the Department of Botany, University of Belgrade - Faculty of Pharmacy.

Extraction solvents

boiling water

Extraction repeats

1

Extraction time

30 min

Extract drying method

freeze-drying

Analysis solvents

aqueous infusion

Detection note

The compound was quantified; the UV spectral data was not shown.

References

S. Samardžić, J. Arsenijević, D. Božić, M. Milenković, V. Tešević, and Z. Maksimović, "Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.," Journal of Ethnopharmacology , vol. 213 , pp. 132–137 , DOI: 10.1016/j.jep.2017.11.013 .