Chemical fact sheet: Naringenin 7-O-glucoside

STD

False

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus tinctorius  L.

cultivated

dried, frozen

Sample note

The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

Detection note

The exact mass obtained.

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

False

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus tinctorius  L.

cultivated

dried, frozen

Sample note

The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

Detection note

The exact mass obtained.

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

False

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus palaestinus  L.

wild

dried, frozen

Sample note

The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

Detection note

The exact mass obtained.

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

False

TLC

False

UV/Vis detector description

Mass spectrometer description

UHPLC-ESI-QTOF-MS, quadrupole time-of-flight

Organism

Carthamus palaestinus  L.

wild

dried, frozen

Sample note

The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.

Drying methods

freeze-dried

Dried material storage temperature

-80 °C

Extraction solvents

70 % methanol

Extraction mass/volume-ratio

25 mg/mL

Extraction repeats

5

Extraction time

35 min

Extraction temperature

4 °C

Analysis solvents

70 % methanol

Detection note

The exact mass obtained.

References

J. Kim, A. Assefa, J. Song, V. Mani, S. Park, S. Lee, K. Lee, D. Kim, and B. Hahn, "Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.," Metabolites , vol. 10 , no. 11 , pp. 440 , DOI: 10.3390/metabo10110440 .

STD

True

TLC

False

UV/Vis detector description

HPLC-DAD

Mass spectrometer description

UHPLC-QTOF-MS

Organism

Salix pyrolifolia

dried buds, fresh, dried

Sample note

The researchers collected S. pyrolifolia samples from different -aged trees of the same clone grown in Kaavi, Finland.

Extraction solvents

cold methanol

Extraction repeats

5

Extract drying method

evaporation in vacuo

Analysis solvents

water:MeOH, 1:1

Detection note

Na+ -adduct = 23.98977; 457.1117-23.98977 = 433.12193; the compound was detected in the barks of all studied ages (1, 3 and 20 years)

References

A. Lavola, M. Maukonen, and R. Julkunen-Tiitto, "Variability in the composition of phenolic compounds in winter-dormant Salix pyrolifolia in relation to plant part and age," Phytochemistry , vol. 153 , pp. 102–110 , DOI: 10.1016/j.phytochem.2018.05.021 .