Analysis result 1
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
623
: ND
|
m/z |
UV/Vis detector description
UHPLC-DAD
Mass spectrometer description
ESI, UHPLC-MS, linear ion trap
Organism
Calendula arvensis
L.
Collection dates
2015-3, 2015-4
Sample note
The samples were identified by Dr. Paolo Silveira. A voucher specimen was deposited in the Herbarium of the Department of Biology University of Aveiro, Portugal.
Drying methods
oven-dried
Extraction solvents
methanol
Extraction mass/volume-ratio
100 mg/mL
Extraction temperature
20±5 °C
Extract drying method
vacuum evaporation
Extract drying temperature
40 °C
References
M.
Faustino,
D.
Pinto,
M.
Gonçalves,
L.
Salgueiro,
P.
Silveira,
and
A.
Silva,
"Calendula L. species polyphenolic profile and in vitro antifungal activity.,"
Journal of Functional Foods
,
vol. 45
,
pp. 254–267
,
DOI: 10.1016/j.jff.2018.04.013
.
Analysis result 2
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
623
: ND
|
m/z |
UV/Vis detector description
UHPLC-DAD
Mass spectrometer description
ESI, UHPLC-MS, linear ion trap
Organism
Calendula suffruticosa
subsp. lusitanica
Collection dates
2015-3, 2015-4
Sample note
The samples were identified by Dr. Paolo Silveira. A voucher specimen was deposited in the Herbarium of the Department of Biology University of Aveiro, Portugal.
Drying methods
oven-dried
Extraction solvents
methanol
Extraction mass/volume-ratio
100 mg/mL
Extraction temperature
20±5 °C
Extract drying method
vacuum evaporation
Extract drying temperature
40 °C
References
M.
Faustino,
D.
Pinto,
M.
Gonçalves,
L.
Salgueiro,
P.
Silveira,
and
A.
Silva,
"Calendula L. species polyphenolic profile and in vitro antifungal activity.,"
Journal of Functional Foods
,
vol. 45
,
pp. 254–267
,
DOI: 10.1016/j.jff.2018.04.013
.
Analysis result 3
| Detection technique |
Values |
Units |
| UV/Vis |
254
268
sh
298
sh
355
|
nm |
| [M⁺ H]⁺ |
625
|
m/z |
UV/Vis detector description
UV
Mass spectrometer description
ESI-MS, ion-trap
Organism
Polygonatum odoratum
(Mill.) Druce
Sample note
The plant was identified by Dr. Enebish Ganbold at the herbarium of the Ulaanbaatar Insitute of Botany, Mongolian Academy of Sciences, where a voucher specimen has been deposited.
Extraction solvents
95% ethanol, water, ethyl acetate
Extraction temperature
20±5 °C
Extract drying method
evaporation under reduced pressure
Detection note
647 [M + Na]+
References
C.
Ganbaatar,
M.
Gruner,
D.
Mishig,
R.
Duger,
A.
Schmidt,
and
H.
Knölker,
"Flavonoid glycosides from the aerial parts of Polygonatum odoratum (Mill.) druce growing in Mongolia,"
The Open Natural Products Journal
,
vol. 8
,
pp. 1–7
,
DOI: 10.2174/1874848101508010001
.
Analysis result 4
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 5
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 6
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 7
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 8
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 9
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 10
UV/Vis detector description
HPLC-DAD, diode array detector
Mass spectrometer description
HPLC-MS, HPLC-MS/MS, triple quadrupole, electrospray, turbo ion spray
Organism
Urtica dioica
'Clone13'
Sample note
The nettle cultivar "Clone" was growing in Prato, Italy at the experimental site. The stalks were frozen in liquid nitrogen and comminuted into a powder. The extraction was performed with 70 % EtOh and pH was adjusted to 3.2 and 2.0 by HCOOH. The raw extract was defatted with n-hexane, dried and adjusted to a final volume with 70 % EtOH.
Extraction solvents
70 % EtOH, HCOOH
Extraction mass/volume-ratio
20 mg/mL
Extraction temperature
20±5 °C
Extract drying method
dried under vacuum
Analysis solvents
70 % EtOH
References
P.
Pinelli,
F.
Ieri,
P.
Vignolini,
L.
Bacci,
S.
Baronti,
and
A.
Romani,
"Extraction and HPLC analysis of phenolic compounds in leaves, stalks, and textile fibers of Urtica dioica L.,"
Journal of Agricultural and Food Chemistry
,
vol. 56
,
pp. 9127–9132
,
DOI: 10.1021/jf801552d
.
Analysis result 11
UV/Vis detector description
HPLC-DAD, diode array detector
Mass spectrometer description
HPLC-MS, HPLC-MS/MS, triple quadrupole, electrospray, turbo ion spray
Organism
Urtica dioica
'Clone13'
Sample note
The nettle cultivar "Clone" was growing in Prato, Italy at the experimental site. The leaves were frozen in liquid nitrogen and comminuted into a powder. The extraction was performed with 70 % EtOH and pH adjusted to 3.2 and 2.0 by HCOOH. The raw extract was defatted with n-hexane, dried and adjusted to a final volume with 70 % EtOH.
Extraction solvents
70 % EtOH, HCOOH
Extraction mass/volume-ratio
20 mg/mL
Extraction temperature
20±5 °C
Analysis solvents
70 % EtOH
References
P.
Pinelli,
F.
Ieri,
P.
Vignolini,
L.
Bacci,
S.
Baronti,
and
A.
Romani,
"Extraction and HPLC analysis of phenolic compounds in leaves, stalks, and textile fibers of Urtica dioica L.,"
Journal of Agricultural and Food Chemistry
,
vol. 56
,
pp. 9127–9132
,
DOI: 10.1021/jf801552d
.
