Analysis result 1
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447
|
m/z |
| MS²⁻ |
284
285
447
|
m/z |
UV/Vis detector description
Mass spectrometer description
ESI-MS/MS
Organism
Rhamnus davurica
Pall.
Sample note
The authentication and identification of the barks of Rhamnus davurica was performed with the researchers of this study which were assisted by the taxonomist Quangwan Hu from Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture (Wuhan Botanicl Garden), Chinese Academy of Sciences. A voucher specimen (No. 0031) was deposited in the herbarium of the Key Laboratory.
Dried material storage temperature
4 °C
Dried material storage notes
the samples were packed in sealed polyethylene bags; stored in a refrigerator until use; dark
Extraction solvents
60 % ethanol
Extraction time
1 h 30 min
Extraction temperature
20±5 °C
Extract drying method
evaporation under reduced pressure
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
References
G.
Chen,
X.
Li,
F.
Saleri,
and
M.
Guo,
"Analysis of flavonoids in Rhamnus davurica and its antiproliferative effects.,"
Molecules
,
vol. 21
,
no. 10
,
pp. 1275
,
DOI: 10.3390/molecules21101275
.
Analysis result 2
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 3
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 4
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 5
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 6
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 7
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 8
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 9
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were yellow and the pistils became orange as the development proceeded, and the colour started to change gradually yellos/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 10
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791)) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to yellow&/red. The pistils became orange as the development proceeded.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 11
| Detection technique |
Values |
Units |
| UV/Vis |
ND
|
nm |
UV/Vis detector description
HPLC-DAD, HPLC-PDA
Mass spectrometer description
LC-ESI-MS
Organism
Filipendula ulmaria
(L.) Maxim.
Sample note
Plant material was identified by Professor Branislava Lakušik (Department of Botany, University of Belgrade - Faculty of Pharmacy) and voucher specimen; number 3872HFF was deposited in the Herbarium of the Department of Botany, University of Belgrade - Faculty of Pharmacy.
Extraction solvents
boiling water
Extract drying method
freeze-drying
Analysis solvents
aqueous infusion
Detection note
The analyses were based on HPLC-UV chromatograms and NMR spectra.
References
S.
Samardžić,
J.
Arsenijević,
D.
Božić,
M.
Milenković,
V.
Tešević,
and
Z.
Maksimović,
"Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. And Filipendula vulgaris Moench.,"
Journal of Ethnopharmacology
,
vol. 213
,
pp. 132–137
,
DOI: 10.1016/j.jep.2017.11.013
.
Analysis result 12
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447
|
m/z |
| MS²⁻ |
284
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Dimitrograd was no. 2-1765. The aerial parts were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z), respectively, are presented from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 13
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447
|
m/z |
| MS²⁻ |
284
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Dimitrograd was no. 2-1765. The bulbs were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z), respectively, are presented from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 14
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447
|
m/z |
| MS²⁻ |
284
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Babusnica was no. 2-1767. The aerial parts were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z), respectively, are presented from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 15
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447
|
m/z |
| MS²⁻ |
284
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Babusnica was no. 2-1767. The bulbs were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z), respectively, are presented from the standard of this compound in the quantitative MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 16
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447
|
m/z |
| MS²⁻ |
285
|
m/z |
UV/Vis detector description
HPLC-UV/Vis
Mass spectrometer description
HPLC/ESI-ITMSn, electrospray ionization multistage ion trap mass spectrometry
Organism
Allium cepa
var. Ramata di Montoro
L.
Sample note
The variety is cultivated in a niche geographical area in southern Italy. The cultivated bulbs were provide by local committee promoters of Cipolla Ramata di Montoro located in Montoro (Campania, Italy).
Extraction solvents
80 % methanol
Extraction mass/volume-ratio
500 mg/mL
Extraction temperature
20±5 °C
Extract drying method
rotary evaporation
Dried extract storage temperature
-20 °C
Analysis solvents
1 % formic acid
References
I.
Tedesco,
V.
Carbone,
C.
Spagnuolo,
P.
Minasi,
and
G.
Russo,
"Identification and quantification of flavonoids from two southern Italian cultivars of Allium cepa L., Tropea (red onion) and Montoro (copper onion), and their capacity to protect human erythrocytes from oxidative stress.,"
Journal of Agricultural and Food Chemistry
,
vol. 63
,
pp. 5229–5238
,
DOI: 10.1021/acs.jafc.5b01206
.
Analysis result 17
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447
: ND
|
m/z |
UV/Vis detector description
HPLC-UV/Vis
Mass spectrometer description
HPLC/ESI-ITMSn, electrospray ionization multistage ion trap mass spectrometry
Organism
Allium cepa
var. Tropea
L.
Sample note
Tropea variety is a well known red onion in southern Italy. The researchers collected onion bulbs in Tropea, Calabria, Italy.
Extraction solvents
80 % methanol
Extraction mass/volume-ratio
500 mg/mL
Extraction temperature
20±5 °C
Extract drying method
rotary evaporation
Dried extract storage temperature
-20 °C
Analysis solvents
1 % formic acid
References
I.
Tedesco,
V.
Carbone,
C.
Spagnuolo,
P.
Minasi,
and
G.
Russo,
"Identification and quantification of flavonoids from two southern Italian cultivars of Allium cepa L., Tropea (red onion) and Montoro (copper onion), and their capacity to protect human erythrocytes from oxidative stress.,"
Journal of Agricultural and Food Chemistry
,
vol. 63
,
pp. 5229–5238
,
DOI: 10.1021/acs.jafc.5b01206
.
Analysis result 18
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447.09131
|
m/z |
| MS²⁻ |
255
284
285
327
|
m/z |
| MS³⁻ |
227
255
|
m/z |
UV/Vis detector description
UHPLC
Mass spectrometer description
UHPLC-MS, HRMS, LTQ OrbiTrap, UHPLC–LTQ OrbiTrap MS/MS, HESI, heated ESI
Organism
Tanacetum parthenium
(L.) Sch. Bip.
Sample note
The samples were collected in Turkey (Taskopru, Karacaoglu village). Taxonomic spotting was performed at Marmara University, Istanbul, Turkey, voucher number: MARE-19056./ Microwave-assisted extraction (MAE) was performed at 600W microwave power.
Extraction solvents
ethanol
Extraction mass/volume-ratio
50 mg/mL
Extract drying method
concentration under vacuum
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
Detection note
MS2 fragments (% base peak): 327 (20), 285 (80), 284 (100), 255 (10); MS3: 255 (100), 227 (10); MS4: 227 (100), 211 (60)
References
G.
Zengin,
A.
Cvetanonović,
U.
Gašić,
A.
Stupar,
G.
Bulut,
I.
Şenkardes,
A.
Dogan,
K.
Sinan,
Z.
Aumeeruddy-Elalfi,
A.
Aktumsek,
and
M.
Mahomoodally,
"Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch. Bip.,"
Industrial Crops and Products
,
vol. 146
,
pp. 112202
,
DOI: 10.1016/j.indcrop.2020.112202
.
Analysis result 19
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447.09131
|
m/z |
| MS²⁻ |
255
284
285
327
|
m/z |
| MS³⁻ |
227
255
|
m/z |
UV/Vis detector description
UHPLC
Mass spectrometer description
UHPLC-MS, HRMS, LTQ OrbiTrap, UHPLC–LTQ OrbiTrap MS/MS, HESI, heated ESI
Organism
Tanacetum parthenium
(L.) Sch. Bip.
Sample note
The samples were collected in Turkey (Taskopru, Karacaoglu village). Taxonomic spotting was performed at Marmara University, Istanbul, Turkey, voucher number: MARE-19056./Sonication of plant-ethanol mixture was done in ultrasonic bath for an hour at 30 °C.
Extraction solvents
ethanol
Extraction mass/volume-ratio
40 mg/mL
Extraction temperature
30 °C
Extract drying method
concentration under vacuum
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
Detection note
MS2 fragments (% base peak): 327 (20), 285 (80), 284 (100), 255 (10); MS3: 255 (100), 227 (10); MS4: 227 (100), 211 (60)
References
G.
Zengin,
A.
Cvetanonović,
U.
Gašić,
A.
Stupar,
G.
Bulut,
I.
Şenkardes,
A.
Dogan,
K.
Sinan,
Z.
Aumeeruddy-Elalfi,
A.
Aktumsek,
and
M.
Mahomoodally,
"Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch. Bip.,"
Industrial Crops and Products
,
vol. 146
,
pp. 112202
,
DOI: 10.1016/j.indcrop.2020.112202
.
Analysis result 20
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
447.09131
|
m/z |
| MS²⁻ |
255
284
285
327
|
m/z |
| MS³⁻ |
227
255
|
m/z |
UV/Vis detector description
UHPLC
Mass spectrometer description
UHPLC-MS, HRMS, LTQ OrbiTrap, UHPLC–LTQ OrbiTrap MS/MS, HESI, heated ESI
Organism
Tanacetum parthenium
(L.) Sch. Bip.
Sample note
The samples were collected in Turkey (Taskopru, Karacaoglu village). Taxonomic spotting was performed at Marmara University, Istanbul, Turkey, voucher number: MARE-19056./The plant samples were macerated at room temperature at dark for 24 h.
Extraction solvents
ethanol
Extraction mass/volume-ratio
50 mg/mL
Extraction temperature
20±5 °C
Extract drying method
concentration under vacuum
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
Detection note
MS2 fragments (% base peak): 327 (20), 285 (80), 284 (100), 255 (10); MS3: 255 (100), 227 (10); MS4: 227 (100), 211 (60)
References
G.
Zengin,
A.
Cvetanonović,
U.
Gašić,
A.
Stupar,
G.
Bulut,
I.
Şenkardes,
A.
Dogan,
K.
Sinan,
Z.
Aumeeruddy-Elalfi,
A.
Aktumsek,
and
M.
Mahomoodally,
"Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch. Bip.,"
Industrial Crops and Products
,
vol. 146
,
pp. 112202
,
DOI: 10.1016/j.indcrop.2020.112202
.
