Analysis result 1
UV/Vis detector description
Mass spectrometer description
Organism
Serratula tinctoria
L.
Sample note
The samples were from Le Jardin Conservatoire des Plantes Tinctoriales.
Extraction solvents
ethanol: water, 3:7, v/v
Extraction mass/volume-ratio
31.3 mg/mL
Extract drying method
concentration under vacuum
References
P.
Guinot,
Gargadennec A,
P.
La Fisca,
A.
Fruchier,
C.
Andary,
and
L.
Mondolot,
"Serratula tinctoria, a source of natural dye: flavonoid pattern and histolocalization.,"
Industrial Crops and Products
,
vol. 29
,
no. 2–3
,
pp. 320–325
,
DOI: 10.1016/j.indcrop.2008.06.007
.
Analysis result 2
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus tinctorius
L.
Sample note
The material (Accession Number 592391) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 3
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The length of spine was very short. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 4
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were orange(-yellow).
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 5
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus palaestinus
L.
Sample note
The material (Accession Number 235663) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These LATE STAGE flower samples were collected when the capitulum begins to expand and the seeds are about to start developing. The florets start to change color to orange/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
Detection note
The exact mass was obtained.
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 6
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea. The collecting (by hand) of of florets of early stage samples started approximately 12 weeks after seed planting. These EARLY STAGE flower samples were collected before the beginning of the flowering, when the upper portion of the florets were about to emerge through the bracts. The samples wer snap-frozen using liquid nitrogen, the freeze--dried and stored at -80 ° until further processing. Visually seen, there was not yet much color in flowers, only white and light yellow hues. The leaf shape was lanceolate.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 7
UV/Vis detector description
Mass spectrometer description
UHPLC-ESI-QTOF-MS, quadrupole time-of-flight
Organism
Carthamus lanatus
L.
Sample note
The material (Accession Number W6 16791) were obtained from USDA National Plant Germplasm system. The seeds were planted and cultivated in a greenhouse at 18-25 °C located at the National Institute of Agricultural Sciences, Jeonju, Korea.These MIDDLE STAGE flower samples were collected when flowering was considered complete, i.e. more than 90 % of the florets were open. The florets were yellow and the pistils became orange as the development proceeded, and the colour started to change gradually yellos/red.
Drying methods
freeze-dried
Dried material storage temperature
-80 °C
Extraction solvents
70 % methanol
Extraction mass/volume-ratio
25 mg/mL
Extraction temperature
4 °C
Analysis solvents
70 % methanol
References
J.
Kim,
A.
Assefa,
J.
Song,
V.
Mani,
S.
Park,
S.
Lee,
K.
Lee,
D.
Kim,
and
B.
Hahn,
"Assessment of metabolic profiles in florets of Carthamus species using ultra-performance liquid chromatography-mass spectrometry.,"
Metabolites
,
vol. 10
,
no. 11
,
pp. 440
,
DOI: 10.3390/metabo10110440
.
Analysis result 8
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
315
|
m/z |
| MS²⁻ |
300
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Dimitrograd was no. 2-1765. The aerial parts were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z) are presented, respectively, from the standard of this compound in the MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 9
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
315
: ND
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Dimitrograd was no. 2-1765. The bulbs were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
Isorhamnetin was not detected in the bulbs, contrary to the aerial parts.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 10
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
315
|
m/z |
| MS²⁻ |
300
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Babusnica was no. 2-1767. The aerial parts were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
The precursor and product ions (m/z) are presented, respectively, from the standard of this compound in the MS/MS-analysis.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 11
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
315
: ND
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-MS/MS, triple-quadrupole mass spectrometer
Organism
Allium flavum
subsp. flavum
L.
Sample note
The whole plants (aerial parts, bulbs) of wild-growing A. flavum subsp. flavum were collected in Serbia. The voucher specimens were prepared, identified and deposited at the Herbarium of the Department of Biology and Ecology (BUNS Herbarium), University of Novi Sad, Faculty of Sciences. The code of the specimens from Babusnica was no. 2-1767. The bulbs were analysed in this group.
Extraction solvents
70 % aqueous methanol
Extraction mass/volume-ratio
125 mg/mL
Extraction temperature
30 °C
Extract drying method
rotary evaporation under vacuum
Extract drying temperature
45 °C
Analysis solvents
70 % aqueous MeOH; 0.5 % formic acid : MeOH (7 : 3)
Detection note
Isorhamnetin was not detected in the bulbs, contrary to the aerial parts.
References
N.
Simin,
D.
Orcic,
D.
Cetojevic-Simin,
N.
Mimica-Dukic,
G.
Anackov,
I.
Beara,
D.
Mitic-Culafic,
and
B.
Bozin,
"Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae),"
LWT - Food Science and Technology
,
vol. 54
,
no. 1
,
pp. 139–146
,
DOI: 10.1016/j.lwt.2013.05.023
.
Analysis result 12
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
315.06380
|
m/z |
| MS²⁻ |
300.03090
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-QTOF-MS/MS
Organism
Tanacetum poteriifolium
Grierson
Sample note
Botanical authentication was d in Turkey.one by the botanist Dr. Ismail Senkardes (Marmara University, Faculty of Pharmacy Turkey). The samples were collected in Kastamonu (Hanonu, Yukaricakircay village)
Drying methods
air-dried, dark
Drying temperature
20±5 °C
Dried material storage temperature
4 °C
Dried material storage notes
10 days
Extraction solvents
methanol
Extraction mass/volume-ratio
50 mg/mL
Extract drying method
evaporation in vacuo
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
Detection note
Methylquercetin may e.g. refer to isorhamnetin (3-methylquercetin), rhamnetin (7-methylquercetin, azaleatin (5-methylquercetin)
References
G.
Zengin,
E.
Sieniawska,
I.
Senkardes,
M.
Picot-Allain,
K.
Sinan,
and
M.
Mahomoodally,
"Antioxidant abilities, key enzyme inhibitory potential and phytochemical profile of Tanacetum poteriifolium Grierson.,"
Industrial Crops and Products
,
vol. 140
,
pp. 111629
,
DOI: 10.1016/j.indcrop.2019.111629
.
Analysis result 13
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
315.06380
|
m/z |
| MS²⁻ |
300.03090
|
m/z |
UV/Vis detector description
Mass spectrometer description
LC-ESI-QTOF-MS/MS
Organism
Tanacetum poteriifolium
Grierson
Sample note
Botanical authentication was d in Turkey.one by the botanist Dr. Ismail Senkardes (Marmara University, Faculty of Pharmacy Turkey). The samples were collected in Kastamonu (Hanonu, Yukaricakircay village)
Drying methods
air-dried, dark
Drying temperature
20±5 °C
Dried material storage temperature
4 °C
Dried material storage notes
10 days
Extraction solvents
ethyl acetate
Extraction mass/volume-ratio
50 mg/mL
Extract drying method
evaporation in vacuo
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
Analysis solvents
ethyl acetate
Detection note
Methylquercetin may e.g. refer to isorhamnetin (3-methylquercetin), rhamnetin (7-methylquercetin, azaleatin (5-methylquercetin)
References
G.
Zengin,
E.
Sieniawska,
I.
Senkardes,
M.
Picot-Allain,
K.
Sinan,
and
M.
Mahomoodally,
"Antioxidant abilities, key enzyme inhibitory potential and phytochemical profile of Tanacetum poteriifolium Grierson.,"
Industrial Crops and Products
,
vol. 140
,
pp. 111629
,
DOI: 10.1016/j.indcrop.2019.111629
.
Analysis result 14
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
315.04993
|
m/z |
| MS²⁻ |
300
301
|
m/z |
| MS³⁻ |
151
227
255
271
283
|
m/z |
UV/Vis detector description
UHPLC
Mass spectrometer description
UHPLC-MS, HRMS, LTQ OrbiTrap, UHPLC–LTQ OrbiTrap MS/MS, HESI, heated ESI
Organism
Tanacetum parthenium
(L.) Sch. Bip.
Sample note
The samples were collected in Turkey (Taskopru, Karacaoglu village). Taxonomic spotting was performed at Marmara University, Istanbul, Turkey, voucher number: MARE-19056./ Microwave-assisted extraction (MAE) was performed at 600W microwave power.
Extraction solvents
ethanol
Extraction mass/volume-ratio
50 mg/mL
Extract drying method
concentration under vacuum
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
Detection note
MS2 fragments (% base peak): 301 (20), 300 (100); MS3: 283 (40), 271 (80), 255 (30), 227 (30), 151 (100); MS4: 107 (100), 83 (15)
References
G.
Zengin,
A.
Cvetanonović,
U.
Gašić,
A.
Stupar,
G.
Bulut,
I.
Şenkardes,
A.
Dogan,
K.
Sinan,
Z.
Aumeeruddy-Elalfi,
A.
Aktumsek,
and
M.
Mahomoodally,
"Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch. Bip.,"
Industrial Crops and Products
,
vol. 146
,
pp. 112202
,
DOI: 10.1016/j.indcrop.2020.112202
.
Analysis result 15
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
315.04993
|
m/z |
| MS²⁻ |
300
301
|
m/z |
| MS³⁻ |
151
227
255
271
283
|
m/z |
UV/Vis detector description
UHPLC
Mass spectrometer description
UHPLC-MS, HRMS, LTQ OrbiTrap, UHPLC–LTQ OrbiTrap MS/MS, HESI, heated ESI
Organism
Tanacetum parthenium
(L.) Sch. Bip.
Sample note
The samples were collected in Turkey (Taskopru, Karacaoglu village). Taxonomic spotting was performed at Marmara University, Istanbul, Turkey, voucher number: MARE-19056./Sonication of plant-ethanol mixture was done in ultrasonic bath for an hour at 30 °C.
Extraction solvents
ethanol
Extraction mass/volume-ratio
40 mg/mL
Extraction temperature
30 °C
Extract drying method
concentration under vacuum
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
Detection note
MS2 fragments (% base peak): 301 (20), 300 (100); MS3: 283 (40), 271 (80), 255 (30), 227 (30), 151 (100); MS4: 107 (100), 83 (15)
References
G.
Zengin,
A.
Cvetanonović,
U.
Gašić,
A.
Stupar,
G.
Bulut,
I.
Şenkardes,
A.
Dogan,
K.
Sinan,
Z.
Aumeeruddy-Elalfi,
A.
Aktumsek,
and
M.
Mahomoodally,
"Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch. Bip.,"
Industrial Crops and Products
,
vol. 146
,
pp. 112202
,
DOI: 10.1016/j.indcrop.2020.112202
.
Analysis result 16
| Detection technique |
Values |
Units |
| [M⁻ H]⁻ |
315.04993
|
m/z |
| MS²⁻ |
300
301
|
m/z |
| MS³⁻ |
151
227
255
271
283
|
m/z |
UV/Vis detector description
UHPLC
Mass spectrometer description
UHPLC-MS, HRMS, LTQ OrbiTrap, UHPLC–LTQ OrbiTrap MS/MS, HESI, heated ESI
Organism
Tanacetum parthenium
(L.) Sch. Bip.
Sample note
The samples were collected in Turkey (Taskopru, Karacaoglu village). Taxonomic spotting was performed at Marmara University, Istanbul, Turkey, voucher number: MARE-19056./The plant samples were macerated at room temperature at dark for 24 h.
Extraction solvents
ethanol
Extraction mass/volume-ratio
50 mg/mL
Extraction temperature
20±5 °C
Extract drying method
concentration under vacuum
Extract drying temperature
40 °C
Dried extract storage temperature
4 °C
Detection note
MS2 fragments (% base peak): 301 (20), 300 (100); MS3: 283 (40), 271 (80), 255 (30), 227 (30), 151 (100); MS4: 107 (100), 83 (15)
References
G.
Zengin,
A.
Cvetanonović,
U.
Gašić,
A.
Stupar,
G.
Bulut,
I.
Şenkardes,
A.
Dogan,
K.
Sinan,
Z.
Aumeeruddy-Elalfi,
A.
Aktumsek,
and
M.
Mahomoodally,
"Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch. Bip.,"
Industrial Crops and Products
,
vol. 146
,
pp. 112202
,
DOI: 10.1016/j.indcrop.2020.112202
.
