Chemical fact sheet: Isatin

STD

True

TLC

False

UV/Vis detector description

HPLC-PDA, photodiode array (PDA)

Mass spectrometer description

HPLC-ESI-MS, ion trap, HPLC-TOF-MS, APCI

Organism

Isatis tinctoria Thüringer Waid  L.

cultivated

ground, dried, cut into small pieces

Collection dates

2003-9

Sample note

The plants were cultivated on the experimental plots of the Agricultural Research Station of Thuringia (TLL), Dornburg, Germany. Specimens of seeds from the strains are kept at the TLL, under accessions number 153/PG 1 (Thüringer Waid).

Drying methods

oven-dried

Drying temperature

40 °C

Dried material storage temperature

15 °C

Dried material storage notes

dark; in the brown glass bottles

Extraction solvents

dichloromethane

Extraction repeats

2

Extraction time

12 min

Extraction temperature

70 °C

Analysis solvents

dichloromethane

Detection note

[M + H]+ 148 (both ESI+ and APCI +)

References

M. Tobias, I. Plitzko, and M. Hamburger, "A comprehensive metabolite profiling of Isatis tinctoria leaf extracts.," Phytochemistry , vol. 70 , no. 7 , pp. 924-934 , DOI: 10.1016/j.phytochem.2009.04.019 .

STD

True

TLC

False

UV/Vis detector description

HPLC-PDA, photodiode array (PDA)

Mass spectrometer description

HPLC-ESI-MS, ion trap, HPLC-TOF-MS, APCI

Organism

Isatis indigotina

cultivated

ground, dried, cut into small pieces

Collection dates

2003-9

Sample note

The plants were cultivated on the experimental plots of the Agricultural Research Station of Thuringia (TLL), Dornburg, Germany. Specimens of seeds from the strains are kept at the TLL, under accessions number 153 PG 12 (I. indigotina).NOTE. There are conflicting views on the taxonomy of I. tinctoria (European woad) and I. indigotica (Chinese woad). I. indigotina was described for the first time by Fortune in 1846, and initially considered as a distinct species. Afterward, some taxonomists have classified it as a variety of I. tinctoria. Nowadays, I. indigotina is considered as a synonym of I. tinctoria [e.g. The Plant List, IPNI (International Plant Name Index), Tropicos]. (Speranzo et al. 2020). In this database, we use the names similarly as the authors.

Drying methods

oven-dried

Drying temperature

40 °C

Dried material storage temperature

15 °C

Dried material storage notes

dark; in the brown glass bottles

Extraction solvents

dichloromethane

Extraction repeats

2

Extraction time

12 min

Extraction temperature

70 °C

Analysis solvents

dichloromethane

Detection note

[M + H]+ 148 (both ESI+ and APCI +)

References

M. Tobias, I. Plitzko, and M. Hamburger, "A comprehensive metabolite profiling of Isatis tinctoria leaf extracts.," Phytochemistry , vol. 70 , no. 7 , pp. 924-934 , DOI: 10.1016/j.phytochem.2009.04.019 .

STD

False

TLC

False

UV/Vis detector description

UV

Mass spectrometer description

HPLC-ESI-MS, HR-ESI-MS/MS

Organism

Isatis tinctoria  L.

wild

dried

Collection dates

2015-6

Sample note

The leaves of Isatis tinctoria L. were collected from Anhui Province in China. They were authenticated by Professor Jin-Cai Lu (Department of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, PR China). A voucher specimen (No. 20160707) is deposited in the Department of Natural Products Chemistry, Shenyang Pharmaceutical University.

Drying methods

air-dried

Extraction solvents

80 % ethanol

Extraction repeats

2

Extraction time

4 h

Extraction temperature

70 °C

Extract drying method

concentration under reduced pressure

References

Z. Xu, Y. Xi, W. Zhou, L. Lou, X. Wang, X. Huang, and S. Song, "Alkaloids and monoterpenes from the leaves of Isatis tinctoria Linnaeus and their chemotaxonomic significance.," Biochemical Systematics and Ecology , vol. 92 , pp. 104089 , DOI: 10.1016/j.bse.2020.104089 .